Visible light responsive flower-like ZnO in photocatalytic antibacterial mechanism towards Enterococcus faecalis and Micrococcus luteus

Publication date: Available online 3 August 2018

Source: Journal of Photochemistry and Photobiology B: Biology

Author(s): Jian-Ai Quek, Sze-Mun Lam, Jin-Chung Sin, Abdul Rahman Mohamed

Abstract

Flower-like ZnO micro/nanostructures were successfully fabricated via a surfactant-free co-precipitation method. The as-synthesized product was characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), UV–vis diffuse reflectance spectroscopy (UV–vis DRS) and photoluminescence (PL) analyses. In the presence of visible light irradiation, the as-synthesized flower-like ZnO showed higher antibacterial activities against Enterococcus faecalis (E. faecalis) and Micrococcus luteus (M. luteus) than that of commercial ZnO. The excellent antibacterial performance of synthesized flower-like ZnO was also observed via the bacterial morphological change, K⁺ ions leakage and protein leakage in extracellular suspension. In addition, the FTIR investigation on both treated bacteria further confirmed the bacterial membrane damage via cellular substance alteration. The enhancement of the antibacterial activity of synthesized ZnO can be attributed to the unique flower-like morphology which can increase the surface OH⁻ groups and the quantity of photogenerated electron-hole pair available to participate in the photocatalytic reaction. The reactive oxidizing species (ROS) scavengers experiments showed that H₂O₂ played a main role in the photocatalytic antibacterial process. Our study showed that the synthesized flower-like ZnO micro/nanostructures can act as efficient antibacterial agents in the photocatalytic antibacterial process under visible light irradiation.