Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice

Journal of Cosmetic Dermatology, EarlyView.

Pigmented transverse nasal band: A distinct presentation

Journal of Cosmetic Dermatology, EarlyView.

Interactions médicamenteuses entre méthotrexate et antibiotiques

Publication date: Available online 28 May 2018
Source:Annales de Dermatologie et de Vénéréologie
Author(s): J.-L. Schmutz

Effects of wind–wave disturbances on adsorption and desorption of tetracycline and sulfadimidine in water–sediment systems

Abstract

Wind–wave disturbances frequently disperse sediment particles into overlying water, which facilitates the adsorption and desorption of contaminants in aquatic ecosystems. Tetracycline (TC) and sulfadimidine (SM2) are common antibiotics that are frequently found in aquatic environments. This study utilized microcosms, comprising sediment and water from Lake Taihu, China, to examine the adsorption and desorption of TC and SM2 under different wind–wave disturbances in a shallow lake environment. The adsorption experiments were conducted with three different concentrations (1, 5, 10 mg/L) of TC and SM2 in the overlying water, and two different (background and strong) wind–wave conditions for 72 h. Subsequently, four microcosms were employed in a 12-h desorption study. Analysis of adsorption progress showed that TC concentration in the overlying water decreased quickly, while SM2 remained almost constant. In the desorption experiments, SM2 released to the overlying water was an order of magnitude greater than TC. These results indicate that sediment particles strongly adsorb TC but weakly adsorb SM2. Compared to background conditions, the strong wind–wave conditions resulted in higher concentrations of TC and SM2 in sediment and facilitated their migration to deeper sediment during adsorption, correspondingly promoting greater release of TC and SM2 from sediment particles into the overlying water during desorption.

Effect of CO 2 concentration on strength development and carbonation of a MgO-based binder for treating fine sediment

Abstract

We previously described a MgO-based binder for treating fine sediment and simultaneously store CO₂. Here, we describe a study of the physical/mechanical characteristics and carbonation reactions of the MgO-based binder used to solidify/stabilize fine sediment in atmospheres containing different CO₂ concentrations. Carbonation of the sediment treated with the MgO-based binder at the atmospheric CO₂ concentration markedly improved the compressive strength of the product. The compressive strength was 4.78 MPa after 365 days of curing, 1.3 times higher than the compressive strength of sediment treated with portland cement. This improvement was caused by the formation of carbonation products, such as hydromagnesite, nesquehonite, and lansfordite, and the constant high pH (~ 12) of the specimen, which favored the growth of hydration products such as calcium silicate hydrates and portlandite. Very low compressive strengths were found when 50 and 100% CO₂ atmospheres were used because of excessive formation of carbonation products, which occupied 78% of the specimen depth. Abundant carbonation products increased the specimen volume and decreased the pH to 10.2, slowing the growth of hydration products. The absence of brucite in specimens produced in a 100% CO₂ atmosphere indicated that MgO carbonation is favored over hydration at high CO₂ concentrations.

Occurrence of PCDD/PCDFs, dioxin-like PCBs, and PBDEs in surface sediments from the Neva River and the Eastern Gulf of Finland (Russia)

Abstract

A total of 26 samples of surface sediments collected in the Neva River (including the St. Petersburg city area) and in the Russian part of the Gulf of Finland were analyzed for 17 polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs), 12 dioxin-like polychlorinated biphenyls (dl-PCBs), and 10 polybrominated diphenyl ethers (PBDEs). The concentrations of total PCDD/Fs and dl-PCBs in sediments ranged from < 0.05 to 219 pg g⁻¹ d.w. and from 44 to 246,600 pg g⁻¹ d.w. respectively. The total World Health Organization toxic equivalent (WHO-TEQ_{PCDD/F + PCB}) values varied between 0.01 and 59.0 pg g⁻¹. In the majority of cases, the PCDD/F and dl-PCB concentrations do not exceed the threshold effect level (TEL) recommended by Helsinki Commission (HELCOM) as quantitative target for the sum of PCDD/Fs and dl-PCBs. The congener profile indicates that combustion is the primary source of PCDD/Fs in most of the studied samples. For the PCBs, the historical use of technical PCB products was identified as the major source. ΣPBDE₁₀ concentrations ranged from 0.004 to 1.8 ng g⁻¹ d.w. The congener profile results show that BDE-47 (tetra-BDE) is the dominant congener in sediment samples. According to the sediment and water quality guidelines established in the EU (PNEC) and in Canada (FEQG), all the samples studied can be classified as lowly contaminated by PBDEs. The sediment concentrations of PCDD/Fs, dl-PCBs, and PBDEs measured in this study were comparable to or lower than those reported for other areas of the Baltic Sea.

mRNA transcript distribution bias between Borrelia burgdorferi bacteria and their outer membrane vesicles

Abstract

Borrelia burgdorferi, a bacterium in the spirochete phylum, is the causative agent of Lyme disease. B. burgdorferi has a linear chromosome with a number of circular and linear plasmids. Bacteria, including B. burgdorferi, release spherical outer membrane vesicles (OMVs) that are known to carry secretory products including metabolites, nucleic acids and proteins. Herein, we provide the first comparative transcriptomic analysis of the vesicles released from B. burgdorferi. We identified a total of ∼1200 unique transcripts with at least one mapped read from the bacterial cell and its OMVs. We compared the spectrum of transcripts between bacterial cell and its OMVs, and found a biased distribution based on the source of transcripts, i.e. plasmid-encoded transcripts are more likely to be enriched in the OMVs. We validated the distribution for some of the transcripts by qPCR. This analysis provides the first evidence that some of the B. burgdorferi transcripts are preferentially packaged in OMV which further suggest that the bacteria might use its OMVs for bacteria-bacteria or bacteria-host communications. This report also suggests a possible involvement of Borrelia-derived OMVs in the development of Lyme disease in both early and post disease syndromes.

Ciprofloxacin binding to GyrA causes global changes in the proteome of Pseudomonas aeruginosa

Abstract

Ciprofloxacin is one of the most widely-used antibiotics, and has proven especially effective at controlling infections associated with the opportunistic human pathogen, Pseudomonas aeruginosa. In this work, we show that sub-inhibitory concentrations of ciprofloxacin induce discrete changes in the intracellular proteome. Central metabolism and cell envelope-associated functions are particularly affected. In spite of the low magnitude of the intracellular proteomic changes, we found that sub-lethal concentrations of ciprofloxacin had substantial effects on motility and exoprotein secretion. Crucially, the proteomic and phenotypic modulations that we observed were absolutely dependent upon the presence of wild-type GyrA; an isogenic strain of P. aeruginosa carrying a ciprofloxacin-insensitive form of GyrA (a T83→I mutant) did not display ciprofloxacin-dependent changes unless complemented with wild-type gyrA in trans. These results show that the diverse effects of sub-inhibitory ciprofloxacin on the cell are routed through its primary target in the cell, DNA gyrase.

Why ‘NOW’?

Abstract

A recently published hypothesis on the nature of time by physicist Robert Muller seeks to provide an objective account of the present moment (the 'now') and the 'flow' of time. Muller also claims that his hypothesis makes testable predictions. It is shown that the predictions offered cannot be used to test Muller's hypothesis, that the hypothesis (as presented) does not rate scientific status, has a number of questionable metaphysical premises, and is merely a re-fashioning of the Growing Block theory of time.

Scientific Progress, Understanding, and Knowledge: Reply to Park

Abstract

Dellsén (2016) has recently argued for an understanding-based account of scientific progress, the noetic account, according to which science (or a particular scientific discipline) makes cognitive progress precisely when it increases our understanding of some aspect of the world. I contrast this account with Bird's (2007, 2015); epistemic account, according to which such progress is made precisely when our knowledge of the world is increased or accumulated. In a recent paper, Park (2017) criticizes various aspects of my account and his arguments in favor of the noetic account as against Bird's epistemic account. This paper responds to Park's objections. An important upshot of the paper is that we should distinguish between episodes that constitute and promote scientific progress, and evaluate account of scientific progress in terms of how they classify different episodes with respect to these categories.

Evaluation of larvicidal, adulticidal, and anticholinesterase activities of essential oils of Illicium verum Hook. f., Pimenta dioica (L.) Merr., and Myristica fragrans Houtt. against Zika virus vectors

Abstract

Aedes aegypti is the vector responsible for transmitting pathogens that cause various infectious diseases, such as dengue, Zika, yellow fever, and chikungunya, worrying health authorities in the tropics. Due to resistance of mosquitoes to synthetic insecticides, the search for more effective insecticidal agents becomes crucial. The aim of this study was to verify the larvicidal, adulticidal, and anticholinesterase activities of the essential oils of the Illicium verum (EOIV), Pimenta dioica (EOPD), and Myristica fragrans (EOMF) against Ae. aegypti. The essential oils (EOs) were obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). The larvicidal and adulticidal activities of EOs were evaluated against third instar larvae and Ae. aegypti adult females, respectively, using the procedures of the World Health Organization (WHO) and the anticholinesterase activity of the EOs by the modified Ellman method. The following major components were identified: (E)-anethole (90.1%) for EOIV, methyl eugenol (55.0%) for EOPD, and sabinene (52.1%) for EOMF. All EOs exhibited larvicidal and adulticidal activity against Ae. aegypti. The highest larval mortality was observed in EOMF with LC₅₀ = 28.2 μg mL⁻¹. Adult mortality was observed after 1 (knockdown) and 24 h exposure, with the highest potential established by the EOIV, KC₅₀ = 7.3 μg mg female⁻¹ and LC₅₀ = 10.3 μg mg female⁻¹. EOIV (IC₅₀ = 4800 μg mL⁻¹), EOMF (IC₅₀ = 4510 μg mL⁻¹), and EOPD (IC₅₀ = 1320 μg mL⁻¹) inhibited AChE. EOMF (4130 μg mL⁻¹) and EOPD (IC₅₀ = 3340 μg mL⁻¹) inhibited BChE whereas EOIV showed no inhibition. The EOs were toxic to larvae and adults of Ae. aegypti, as well as being less toxic to humans than the currently used insecticides, opening the possibility of elaboration of a natural, safe, and ecological bioinsecticide for vector control.

Use of a combination of in vitro models to investigate the impact of chlorpyrifos and inulin on the intestinal microbiota and the permeability of the intestinal mucosa

Abstract

Dietary exposure to the organophosphorothionate pesticide chlorpyrifos (CPF) has been linked to dysbiosis of the gut microbiota. We therefore sought to investigate whether (i) CPF's impact extends to the intestinal barrier and (ii) the prebiotic inulin could prevent such an effect. In vitro models mimicking the intestinal environment (the SHIME®) and the intestinal mucosa (Caco-2/TC7 cells) were exposed to CPF. After the SHIME® had been exposed to CPF and/or inulin, we assessed the system's bacterial and metabolic profiles. Extracts from the SHIME®'s colon reactors were then transferred to Caco-2/TC7 cultures, and epithelial barrier integrity and function were assessed. We found that inulin co-treatment partially reversed CPF-induced dysbiosis and increased short-chain fatty acid production in the SHIME®. Furthermore, co-treatment impacted tight junction gene expression and inhibited pro-inflammatory signaling in the Caco-2/TC7 intestinal cell line. Whereas, an isolated in vitro assessment of CPF and inulin effects provides useful information on the mechanism of dysbiosis, combining two in vitro models increases the in vivo relevance.

Chromium (VI) biosorption by Saccharomyces cerevisiae subjected to chemical and thermal treatments

Abstract

The potential of chemically and thermally treated Saccharomyces cerevisiae as biosorbents for chromium (VI) was investigated in this work. The presence of this toxic metal in industrial effluents is harmful to the environment, so, it is important to develop environmental friendly methods for Cr(VI) removal from these effluents. Biosorption using microorganisms such as S. cerevisiae is a viable treatment option because this biomass is easily available as a residue of fermentation industries. In this study, the affecting variables on Cr(VI) biosorption were studied by constructing biosorption isotherms, using lyophilized yeast subjected to chemical and thermal treatments. S. cerevisiae was able to remove 99.66% of Cr(VI) from effluents by biosorption. The significant variables affecting biosorption were pH, initial Cr(VI) concentration, and contact time. The biosorption isotherms were represented by the Freundlich model for the untreated biomass, BET model for the chemically treated biomass, and Langmuir model for the heat-treated biomass. Thermal treatment increased the biosorption affinity of the biomass for chromium, while the chemical treatment facilitated the formation of a multilayer.

Biocompatible metal decontamination from soil using Ageratum conyzoides

Abstract

Metal pollution in soil is a serious problem among waste landfill sites and associated environment all over the globe. Amelioration of contaminated soil by plant bioaccumulation is an important strategy to protect the soil environment. Ageratum conyzoides is a common weed species that can grow easily in any contaminating site and bioaccumulate heavy metals present in the e-waste dumping/recycling sites as a natural scavenger. Soil selected for the study was contaminated with waste cathode ray tube (CRT) and printed circuit board (PCB) powder in the concentration range of 1–10 g/kg. Soil decontamination was achieved by using weed plants with ethylene diamine tetraacetic acid (EDTA, 0.1 g/kg) and kinetin (100 μM) combination in pot experiments. Fe, Mn, Zn, and Cu accumulation was found to be highest in leaves (6.51–38.58; 0.14–73.12; 5.24–269.07; 9.38–116.59%); Pb and Cr in stem (22.83–113.41; 21.05–500%), respectively, as compared with blank. Ion chromatography was used as a tool for the measurement of essential ions present in plant under different conditions. Plants showed better growth in terms of shoot, root length, biomass weight, and chlorophyll content with the proposed combination. EDTA allows the metals available for the accumulation through possible complexation. Also, the compatibility of kinetin to manage stress in plant is found to be enhanced in the presence of EDTA due to possible π-π interaction. Metal stress condition causes the deficiency of essential ions in the plants thereby disturbing its biochemistry and results in its eventual death. EDTA-kinetin hybrid treatment was found to be compatible for metal decontamination from soil, its detoxification in plants by changing its environment and restoring the essential ions for the survival of plant.

Numerical-ecotoxicological approach to assess potential risk associated with oilfield production chemicals discharged into the sea

Abstract

Several different chemical products are used on oil platforms to aid oil-water separation during the production process. These chemicals may enter into the sea by means of production water (PW), the main discharge derived from oil and gas offshore platforms. Consequently, toxic effects may occur in the marine environment, causing reductions in wildlife numbers, degrading ecosystem functions and threatening human health. For most of these chemicals, environmental toxicity and safety thresholds in marine ecosystems have not been fully investigated as yet. In this work, a numerical-ecotoxicological approach is proposed to assess the potential environmental risk associated with the discharge of five oilfield production chemicals (deoiler, scale inhibitor, corrosion inhibitor, catalyst, dehydrating agent) from a platform in the southern Adriatic Sea (Mediterranean Sea). Their concentrations in the seawater are numerically predicted, under different seasonal conditions, starting from the real concentrations used during the production process. The predicted concentrations are then evaluated in terms of possible toxic effects in order to assess the potential risk of oilfield production chemicals discharged into the sea.

pH-dependent microbial reduction of uranium(VI) in carbonate-free solutions: UV-vis, XPS, TEM, and thermodynamic studies

Abstract

U(VI)_aq bioreduction has an important effect on the fate and transport of uranium isotopes in groundwater at nuclear test sites. In this study, we focus on the pH-dependent bioreduction of U(VI)_aq in carbonate-free solutions and give mechanistic insight into the removal kinetics of U(VI)_aq. An enhancement in the removal of U(VI)_aq with increasing pH was observed within 5 h, e.g., from 19.4% at pH 4.52 to 99.7% at pH 8.30. The removal of U(VI)_aq at pH 4.52 was due to the biosorption of U(VI)_aq onto the living cells of Shewanella putrefaciens, as evidenced by the almost constant UV-vis absorption intensity of U(VI)_aq immediately after contact with S. putrefaciens. Instead, the removal observed at pH 5.97 to 8.30 resulted from the bioreduction of U(VI)_aq. The end product of U(VI)_aq bioreduction was analyzed using XPS and HRTEM and identified as nanosized UO₂. An increasing trend in the biosorption of U(VI)_aq onto heat-killed cells was also observed, e.g., ~ 80% at pH 8.38. Evidently, the U(VI)_aq that sorbed onto the living cells at pH > 4.52 was further reduced to UO₂, although biosorption made a large contribution to the initial removal of U(VI)_aq. These results may reveal the removal mechanism, in which the U(VI)_aq that was sorbed onto cells rather than the U(VI)_aq complexed in solution was reduced. The decreases in the redox potentials of the main complex species of U(VI)_aq (e.g., \( {\left(\mathrm{U}{\mathrm{O}}_2\right)}_3{\left(\mathrm{OH}\right)}_7^{-} \) and \( {\left(\mathrm{U}{\mathrm{O}}_2\right)}_4{\left(\mathrm{OH}\right)}_7^{+} \) ) with increasing pH support the proposed removal mechanism.

Bacteria enhanced lignocellulosic activated carbon for biofiltration of bisphenols in water

Abstract

There are eight bisphenol analogues being identified and characterized; among them, bisphenol A (BPA) is on the priority list on the basis of its higher level of uses, occurrence, and toxicity. The endocrine system interfered by BPA has been inventoried as it has the same function as the natural hormone 17β-estradiol and binds mainly to the estrogen receptor (ER) to exhibit estrogenic activities. The BPA concentration in surface waters (14–1390 ng/L) in many parts of the world, such as Japan, Korea, China, and India, was also a significant concern. Research efforts are focusing on restricting BPA consumption as well as removing BPA in our environment especially in drinking water. Current opinion is that lignocellulosic activated carbon stimulated with BPA-degrading bacteria could have the potential to provide solution for recent challenges faced by water utilities arising from BPA contamination in water. This technology has some new trends in the low-cost biofiltration process for removing BPA. This review is to provide in-depth discussion on the fate of BPA in our ecosystem and underlines methods to enhance the efficacy of activated carbon in the presence of BPA-degrading bacteria in the biofiltration process.

Characterization of drilling waste from shale gas exploration in Central and Eastern Poland

Abstract

The purpose of this research was to determine and evaluate the chemical properties of drilling waste from five well sites in Central and Eastern Poland. It was found that spent drilling fluids can contain high values of nickel and mercury (270 and 8.77 mg kg⁻¹_, respectively) and can exceed the maximum permissible limits recommended by the EC regulations for safety of soils (75 mg kg⁻¹ for nickel and 1.5 mg kg⁻¹ for mercury). The heavy metal concentrations in the studied drill cuttings did not exceed the maximum permissible limits recommended by the EC regulation. Drilling wastes contain macroelements (e.g., calcium, magnesium, and potassium) as well as trace elements (e.g., copper, iron, zinc, and manganese) that are essential for the plant growth. It was stated that water extracts of drilling fluids and drill cuttings, according to anions presence, had not any specific constituents of concern based on FAO irrigation guidelines, the USEPA WQC, and toxicity values. X-ray diffraction analysis was used to understand the structure and texture of waste drilling fluid solids and drill cuttings. Analysis of the mineralogical character of drilling fluid solids revealed that they contained calcite, quartz, muscovite, sylvite, barite, dolomite, and orthoclase. Drill cuttings contained calcite quartz, muscovite, barite, dolomite, and barium chloride.

Short-term effects of ambient air pollutants and myocardial infarction in Changzhou, China

Abstract

Ambient air pollution had been shown strongly associated with cardiovascular diseases. However, the association between air pollution and myocardial infarction (MI) is inconsistent. In the present study, we conducted a time-series study to investigate the association between air pollution and MI. Daily air pollutants, weather data, and MI data were collected from January 2015 to December 2016 in Changzhou, China. Generalized linear model (GLM) was used to assess the immediate effects of air pollutants (PM_2.5, PM₁₀, NO₂, SO₂, and O₃) on MI. We identified a total of 5545 cases for MI, and a 10-μg/m³ increment in concentrations of PM_2.5 and PM₁₀ was associated with respective increases of 1.636% (95% confidence interval [CI] 0.537–2.740%) and 0.805% (95% CI 0.037–1.574%) for daily MI with 2-day cumulative effects. The associations were more robust among males and in the warm season versus the cold one. No significant effect was found in SO₂, NO₂, or O₃. This study suggested that short-term exposure to PM_2.5 and PM₁₀ was associated with the increased MI risks. Our results might be useful for the primary prevention of MI exacerbated by air pollutants.