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Τρίτη 21 Νοεμβρίου 2017

Abstraction in ecology: reductionism and holism as complementary heuristics

Abstract

In addition to their core explanatory and predictive assumptions, scientific models include simplifying assumptions, which function as idealizations, approximations, and abstractions. There are methods to investigate whether simplifying assumptions bias the results of models, such as robustness analyses. However, the equally important issue – the focus of this paper – has received less attention, namely, what are the methodological and epistemic strengths and limitations associated with different simplifying assumptions. I concentrate on one type of simplifying assumption, the use of mega parameters as abstractions in ecological models. First, I argue that there are two kinds of mega parameters qua abstractions, sufficient parameters and aggregative parameters, which have gone unnoticed in the literature. The two are associated with different heuristics, holism and reductionism, which many view as incompatible. Second, I will provide a different analysis of abstractions and the associated heuristics than previous authors. Reductionism and holism and the accompanying abstractions have different methodological and epistemic functions, strengths, and limitations, and the heuristics should be viewed as providing complementary research perspectives of cognitively limited beings. This is then, third, used as a premise to argue for epistemic and methodological pluralism in theoretical ecology. Finally, the presented taxonomy of abstractions is used to comment on the current debate whether mechanistic accounts of explanation are compatible with the use of abstractions. This debate has suffered from an abstract discussion of abstractions. With a better taxonomy of abstractions the debate can be resolved.



Association of oxidative stress and dynamic thiol-disulphide homeostasis with atopic dermatitis severity and chronicity in children: a prospective study

Summary

Background

Oxidative stress (OS) has an important effect on the pathogenesis of atopic dermatitis (AD). Thiols are antioxidants that regulate intracellular redox metabolism and protect keratinocytes against OS damage in the stratum corneum.

Aim

To investigate dynamic thiol-disulphide homeostasis (dTDH) as a novel OS parameter in children with AD, and its relationship with disease severity and chronicity.

Methods

Severity of AD was determined by using the instruments SCORing Atopic Dermatitis (SCORAD) and Eczema Area And Severity Index (EASI) upon enrolment in the study (SCORAD1 and EASI1) and after 1 year (SCORAD2 and EASI2). Native thiol, total thiol and disulphide levels were measured as novel OS parameters, and the ratios of disulphide/native thiol, disulphide/total thiol and native/total thiol were calculated as dTDH.

Results

In the AD group, the serum disulphide level and the ratios of disulphide/native thiol and disulphide/total thiol were significantly lower than in healthy controls (P = 0.01, P < 0.01 and P < 0.01, respectively). There was no significant association between OS parameters and disease severity (P > 0.05). SCORAD2 and EASI2 were positively correlated with disulphide/native thiol ratio (r = 0.29, P < 0.03 and r = 0.35, P < 0.01, respectively), whereas they were negatively correlated with the native/total thiol ratio (r = −0.30, P = 0.02 for both).

Conclusions

Both OS and impaired dTDH were found to be related to childhood AD. None of the OS parameters was associated with AD severity. dTDH is a possible diagnostic tool to predict AD chronicity.



Differential Perturbation of the Protrotropic Equilibrium of a Biological Photosensitizer within Bile Salt Aggregates of Varying Hydrophobicity: A Fluorimetric Investigation

Abstract

The present work reveals the binding interactions of a credible cancer cell photosensitizer, harmane (HM) with some selected bile salt aggregates of dissimilar hydrophobicity viz. sodium deoxycholate (NaDC), sodium cholate (NaC) and sodium taurocholate (NaTC). The explicit variation of the prototropic equilibrium of the photosensitizer both in the ground and excited state has been utilized to scrutinize the interaction phenomena. Differential modulation in the prototropic equilibrium of HM in the aforesaid aggregates has been explained on the basis of the structural dissimilarities of the bile-salt monomers. The contrived hydrophobic surroundings provided by the aggregates have been reflected on the spectroscopic results; especially in the time-resolved fluorescence and the rotational dynamical behavior of the molecule of interest. Slow solvent reorientation time with regard to the lifetime of HM proliferated by the Red Edge Effect in two specific bile-salts namely NaC and NaTC; whereas its absence in NaDC aggregates has also been elucidated on the basis of accessibility of the solvent molecules within the aggregates.

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Open-label study assessing the efficacy and tolerability of topical skincare and sun protection products following intense pulsed light treatment

Summary

Background

The visible signs of photodamage can be improved by intense pulsed light (IPL). Active ingredients in cosmeceuticals also have effects on skin quality and pigmentation, and can camouflage post-treatment side effects. Combination therapies utilizing different treatment modalities have been shown to optimize clinical outcomes for skin rejuvenation and patient satisfaction.

Aim

To evaluate the efficacy of a combination of IPL with a daily topical skincare and sunscreen regimen for the treatment of facial photodamage and for the improvement of IPL treatment tolerability.

Patients/Methods

Twenty female subjects with moderate-to-severe facial photodamage, with past history of IPL treatments, received one IPL treatment followed by the use of the topical skincare regimen for 8 weeks. An investigator assessed facial photodamage and hyperpigmentation at baseline, week 4, and week 8, and postprocedure erythema. Subject questionnaires were also administered at each visit.

Results

Compared to baseline, there was a significant improvement in photodamage and hyperpigmentation of bare facial skin. The application of the skincare regimen resulted in a significant reduction in post-IPL erythema, stinging/burning, and itching. The majority of patients were very satisfied or satisfied and felt the treatment regimen improved various aspects of skin quality and the tolerability of the procedure.

Conclusions

The addition of a topical skincare regimen after IPL treatment to the face resulted in significant improvements in facial photodamage and pigmentation, decreased post-treatment side effects, and increased tolerability.



Comparative study of buffered 50% glycolic acid (pH 3.0) + 0.5% salicylic acid solution vs Jessner's solution in patients with acne vulgaris

Summary

Background

Superficial chemical peels are frequently used in acne vulgaris treatment. Although glycolic acid (GA) has been widely used in clinical practice, its pH ranges from 0.08-2.75 and thus should be neutralized after application to avoid burns.

Objective

To evaluate treatment efficacy and safety of chemical peeling using buffered 50% GA (pH 3.0) + 0.5% salicylic acid (SA) solution that does not need to be neutralized in the treatment of acne vulgaris compared to the conventional peeling using Jessner's solution.

Methods

We performed a prospective, randomized, evaluator-blind, split-face clinical trial. Twenty patients were randomized by assigning one side of each patient's face to receive a 50% GA (pH 3.0) + 0.5% SA peel (GA side) and the other side to receive the Jessner's solution (Jessner's solution side). All patients underwent 2 sessions of treatment spaced 2 weeks apart. Lesion count, acne severity, subjective efficacy assessment, and side effects were evaluated.

Results

The total lesion count was significantly reduced for the GA and Jessner's solution sides (< .001). However, there was no significant difference in the total lesion count, acne severity, or subjective efficacy assessment between the 2 sides (> .05). The GA side had fewer side effects than the Jessner's solution side.

Conclusion

The results of this study suggest that chemical peeling using the 50% GA (pH 3.0) + 0.5% SA solution can be as effective and convenient as the conventional peeling using Jessner's solution in the treatment of acne vulgaris and may show fewer adverse events than the conventional peeling.



Contact dermatitis caused by Geranium robertianum with erythema multiforme-like reaction



Christmas tree dermatitis caused by colophonium allergy



Leukoderma following allergic contact dermatitis caused by the silicone component silprene-30A/B in swimming goggles



Allergic contact dermatitis caused by acrylic acid used in transcutaneous electrical nervous stimulation



Thank you to reviewers



Allergic contact dermatitis caused by polymethylmethacrylate following intradermal filler injection



Hair colour shampoo dermatitis



Acetophenone azine: a new shoe allergen causing severe foot dermatitis



A case of contact dermatitis syndrome caused by Dermabond®, followed by contact dermatitis caused by false eyelash glue and Aron Alpha® glue: possibility of cross-reactions among cyanoacrylates



Erratum



Acrylate allergy: time to intervene



Allergic contact dermatitis caused by 2-ethyl cyanoacrylate contained in glucose sensor sets in two diabetic adults



Severe stomatitis with a deep buccal ulcer associated with an allergic reaction to methyl methacrylate used for dental treatment



Contact urticaria caused by a silk bed quilt in a young atopic female



Polyhexamethylene biguanide and alkyl glucosides: unexpected allergens in an antimicrobial foam dressing



Re: Protein contact dermatitis caused by lime in a pastry chef (Contact Dermatitis 2015: 73: 54–56)



Immunoglobulin G; structure and functional implications of different subclass modifications in initiation and resolution of allergy

Abstract

IgE and not IgG is usually associated with allergy. IgE lodged on mast cells in skin or gut and basophils in the blood allows for the prolonged duration of allergy through the persistent expression of high affinity IgE receptors. However, many allergic reactions are not dependent on IgE and are generated in the absence of allergen specific and even total IgE. Instead, IgG plasma cells are involved in induction of, and for much of the pathogenesis of, allergic diseases. The pattern of IgG producing plasma cells in atopic children and the tendency for direct or further class switching to IgE are the principle factors responsible for long-lasting sensitization of mast cells in allergic children. Indirect class switching from IgG producing plasma cells has been shown to be the predominant pathway for production of IgE while a Th2 microenvironment, genetic predisposition, and the concentration and nature of allergens together act on IgG plasma cells in the atopic tendency to undergo further immunoglobulin gene recombination. The seminal involvement of IgG in allergy is further indicated by the principal role of IgG4 in the natural resolution of allergy and as the favourable immunological response to immunotherapy. This paper will look at allergy through the role of different antibodies than IgE and give current knowledge of the nature and role of IgG antibodies in the start, maintenance and resolution of allergy.

Thumbnail image of graphical abstract

The induction, the pathology, and the resolution of allergy involve antibodies other than specific IgE to allergens. Allergy depends on the pattern of IgG producing plasma cells in atopic children and the tendency for direct or further class switching from IgG to IgE, resulting in the sensitising of mast cells in allergic children.



Speckled acral hypopigmentation in an adolescent

Abstract

We report a case of speckled acral hypopigmentation in a 12-year-old girl. She presented with asymptomatic hypopigmented macules on the hands and feet. This rare entity is a proposed variant of reticulate acropigmentation and of unknown etiology.



Urticarial vasculitis after meningococcal serogroup B vaccine in a 6-year-old girl

Abstract

The first vaccine that shows significant potential in providing broad coverage against serogroup B meningococcal disease has recently been approved. Because of its newness, potential adverse events need to be reported. Here we report a case of urticarial vasculitis, a rare disease in children, in probable relationship with the novel vaccine.



Erythromelanosis follicularis faciei et colli: A clinicoepidemiologic study

Abstract

We describe 25 cases of erythromelanosis follicularis faciei et colli from India. The male:female ratio was 5.25:1 and the average age of onset was 12.3 years. The cheeks, preauricular area, and submandibular region were the sites most commonly affected. Keratosis pilaris was seen in 22 (88%) of the patients.



IDSA POSITION STATEMENT: Why IDSA Did Not Endorse the Surviving Sepsis Campaign Guidelines

Abstract
IDSA did not endorse the 2016 Surviving Sepsis Campaign Guidelines despite being represented in the working group that drafted the guidelines document. Leadership from IDSA, the Surviving Sepsis Campaign Guidelines, and the Society of Critical Care Medicine had numerous amicable discussions primarily regarding the bolded, rated guidelines recommendations. Our societies had different perspectives, however, regarding the interpretation of the major studies that informed the guidelines' recommendations thus leading us to different conclusions and different perspectives on the recommendations. IDSA consequently elected not to endorse the guidelines. IDSA nonetheless hopes to be able to continue collaborating with the Surviving Sepsis Campaign and the Society of Critical Care Medicine to resolve our differences and to develop further strategies together to prevent sepsis and septic shock as well as reduce death and disability from these conditions both nationally and globally.

A two-centre randomised trial of an additional early dose of measles vaccine: Effects on mortality and measles antibody levels

Abstract
Background
Besides protecting against measles, measles vaccine (MV) may have beneficial non-specific effects. We tested the effect of an additional early MV on mortality and measles antibody levels.
Methods
Children aged 4-7 months in two rural health and demographic surveillance sites in Burkina Faso and Guinea-Bissau were randomised 1:1 to an extra early standard dose of MV (Edmonston-Zagreb strain) or no extra MV 4 weeks after the third diphtheria-tetanus-pertussis-hepatitis B-Haemophilus-influenzae-type-b vaccine. All children received routine MV at 9 months. We assessed mortality through home visits and compared mortality from enrolment to 3 years of age in Cox proportional hazards models, censoring for subsequent non-trial MV. Subgroups of participants had blood sampled at enrolment, before the 9 months MV and in the second year of life to assess measles antibody level.
Results
Among 8309 children enrolled July 18, 2012-December 3, 2015, we registered 145 deaths (mortality rate: 16/1000 person-years). The mortality was lower than anticipated and did not differ by randomisation group (hazard ratio=1.05 (95%CI: 0.75-1.46)).At enrolment, 4% (16/447) of children in Burkina Faso and 21% (90/422) in Guinea-Bissau had protective measles antibody levels. By 9 months of age, no measles-unvaccinated/unexposed child had protective levels, while 92% (306/333) of early MV recipients had. At final follow-up, 98% (186/189) in the early MV group and 97% (196/202) in the control group had protective levels.
Conclusion
Early MV did not reduce all-cause mortality. Most children were susceptible to measles infection at 4-7 months and responded with high antibody levels to early MV.

Rigorous Clinical Trial Design in Public Health Emergencies Is Essential

Abstract
Randomized clinical trials are the most reliable approaches to evaluating the effects of new treatments and vaccines. During the 2014-15 West African Ebola epidemic, many argued that such trials were neither ethical nor feasible in an environment of limited health infrastructure and severe disease with a high fatality rate. Consensus among the numerous organizations providing help to the affected areas was never achieved, resulting in fragmented collaboration, delayed study initiation, and ultimately failure to provide definitive evidence on the efficacy of treatments and vaccines. Randomized trials were in fact approved by local ethics boards and initiated, demonstrating that randomized trials, even in such difficult circumstances, are feasible. Improved planning and collaboration among research and humanitarian organizations, and affected communities, in the interepidemic periods are needed to ensure that questions regarding the efficacy of vaccines and treatments can be definitively answered during future public health emergencies.

Immunogenicity and protection from a single dose of internationally available killed oral cholera vaccine: a systematic review and meta-analysis

Abstract
In addition to improved water supply and sanitation, the two-dose killed oral cholera vaccine (OCV) is an important tool for the prevention and control of cholera. We aimed to document the immunogenicity and protection (efficacy and effectiveness) conferred by a single OCV dose against cholera. The meta-analysis showed an estimated 73% and 77% of individuals seroconverted to the Ogawa and Inaba serotypes, respectively, after an OCV first dose. The estimates of single-dose vaccine protection from available studies are 87% at 2 months decreasing to 33% at 2 years. Current immunologic and clinical data suggest that protection conferred by a single dose of killed OCV may be sufficient to reduce short-term risk in outbreaks or other high-risk settings, which may be especially useful when vaccine supply is limited. However, until more data suggests otherwise, a second dose should be given as soon as circumstances allow to ensure robust protection.

Identification determinant factors on willingness to pay for health services in Iran

A common method used to examine the relationship between internal preferences and caring externalities is willingness to pay (WTP) approach. We aimed to estimate WTP for health status with different severity l...

Deciphering psoriasis. A bioinformatic approach

Psoriasis is an immune-mediated, inflammatory and hyperproliferative disease of the skin and joints. The cause of psoriasis is still unknown. The fundamental feature of the disease is the hyperproliferation of keratinocytes and the recruitment of cells from the immune system in the region of the affected skin, which leads to deregulation of many well-known gene expressions.

Environmental mineralization of caffeine micro-pollutant by Fe-MFI zeolites

Abstract

Environmentally emerging micro-pollutant, caffeine, was mineralized (i.e., full degradation) by the isomorphic incorporation of Fe into silicalite-1 (mordenite framework inverted (MFI) structure zeolite) through a microwave synthesis method. The Fe incorporation conferred mesopore formation that facilitated caffeine access and transport to the MFI zeolite structure. Increasing the Fe content favored the formation of Fe(O)4 sites within the MFI structure. The catalytic activity for the degradation of caffeine increased as a function of Fe(O)4 sites via a Fenton-like heterogeneous reaction, otherwise not attainable using Fe-free pure MFI zeolites. Caffeine degradation reached 96% (TOC based) for zeolites containing 2.33% of Fe.



Assessment of pollutions and identification of sources of heavy metals in sediments from west coast of Shenzhen, China

Abstract

The sediment samples were collected from eight sites located in the Pearl River Estuary and the Shenzhen Bay of the west coast of Shenzhen. The distributions of the seven elements Zn, Cr, Hg, Cu, Cd, Pb and As have been analyzed, and their pollution degrees, corresponding potential ecological risks and source identifications have been studied using geo-accumulation index, potential ecological risk index and integrated multivariate statistical methods, respectively. Based on the calculated geo-accumulation indices, the contamination levels of all elements in the Pearl River Estuary are similar to those in the Shenzhen Bay, reflecting that these elements in the study areas have similar sources because of the adequate seawater exchange. The calculated potential ecological risk indices suggest that Cd and Hg are at considerable and moderate risk, respectively. Multivariate statistical analyses further reveal that Zn, Hg, Cd and Pb originated from industrial wastewater, while Cr and Cu are mainly from both industrial wastewater and agricultural sources, and As is mainly from natural source. These research results provide baseline information for both the coastal environment management and the worldwide heavy metal distribution and assessment.



Solid fuel use for cooking and its health effects on the elderly in rural China

Abstract

Indoor air pollution is mainly caused by solid fuel use for cooking in developing countries. Many previous studies focused on its health risks on the children and in specific local area. This paper investigates household energy usage and transition for cooking in rural China and the health effects on the elderly. A national large-scale dataset CHARLS (China Health and Retirement Longitudinal Study) covering 450 villages and communities is employed. Logit regressions were used to quantitatively estimate the effects, after controlling for some factors such as income, demographic, and geographical variables. The results robustly show that compared to non-solid fuels, solid fuel use significantly increases the possibility of chronic lung diseases (30%), exacerbation of chronic lung diseases (95%), seizure of heart disease (1.80 times), and decreases self-evaluated health status of the elderly (1.38 times). Thus, it is urgent to improve clean energy access for cooking in rural China.



Assessment of water resource contamination by pesticides in vegetable-producing areas in Burkina Faso

Abstract

This study proposes a comprehensive approach to investigate water resource contamination by pesticides under the specific climatic and hydrological conditions of the Sudano-Sahelian climate. Samples were collected from traditional wells, boreholes, and a lake in Burkina Faso. A multiresidue analysis was developed for 25 pesticides identified during field surveys. Polar organic chemical integrative samplers (POCIS) were used to confirm trends observed with grab samples. Uptake kinetics of POCIS were assessed by in situ calibration. The proposed use of nonlinear least squares regression proved to be a robust approach for estimating time-weighted average concentration in cases of nonlinear uptake. High sampling rates for triazines in a quasi-stagnant lake were attributed to warm water temperatures (30.8 ± 1.3 °C). The combination of sampling techniques during a 3-year monitoring period allowed for identifying potential interactions between resources, seasonal patterns, and origins of contaminants. Atrazine, azadirachtin, carbofuran, chlorpyrifos, cypermethrin, dieldrin, imidacloprid, and profenofos exceeded 0.1 μg L−1, indicating a potential risk for the consumers. Ecological risk assessment was performed using the Pesticide Tool Index for fish, cladocerans, and benthic invertebrates. Peak concentrations of cypermethrin and chlorpyrifos were systematically associated with hazard to the studied taxa.



Kinetic simulation and prediction of pyrolysis process for non-metallic fraction of waste printed circuit boards by discrete distributed activation energy model compared with isoconversional method

Abstract

Kinetic studies on the pyrolysis process for non-metallic fraction (NMF) of waste printed circuit boards (WPCBs) were conducted using both the isoconversional SKAS method and the discrete distributed activation energy model (discrete DAEM). The pyrolysis process of the NMF sample could be classified into three stages, and a large mass loss was observed from 98 to 570 °C, attributed to thermal degradation of epoxy resins in the NMF sample. The kinetic parameters, including activation energies (E i), pre-exponential factors (A i), and contributed fractions (f i,0), were determined. It indicated that the discrete DAEM could predict the pyrolysis process of the NMF more accurately and completely when compared with the isoconversional SKAS method. In the discrete DAEM, E i and A i values were evaluated at 99 equally spaced intervals of conversion. The E i and A i (in the form of lnA i) transformed with reaction progress, ranging from 80.9 to 240.5 kJ/mol and 19.07 to 39.55 s−1, respectively, with the conversion increased from 0.01 to 0.99. The pyrolysis of the NMF of WPCBs could be accurately characterized as 17 dominating reactions from f i,0 results.

Graphical abstract



Bioagents and silicon promoting fast early upland rice growth

Abstract

Upland rice can overcome major challenges through the insertion of silicate fertilization and the presence of plant growth-promoting microorganisms (PGPMs) during its cultivation, as these factors promote an increase in vigor and plant disease resistance. Two consecutive experiments were conducted to evaluate the beneficial effects of silicon fertilization combined with the PGPM, Pseudomonas fluorensces, Burkholderia pyrrocinia, and a pool of Trichoderma asperellum, in upland rice seedlings, cultivar BRS Primavera CL: (a) E1, selecting PGPM type and Si doses for rice growth promotion and leaf blast supression, and (b) E2, evaluating physiological characteristics correlated with mechanisms involved in the higher vegetative growth in highlighted treatments from E1. In E1, 2 Si t ha−1 combined with the application of T. asperellum pool or PGPM mixture increased 54% in root dry matter biomass and 35 and 65% in shoot and root lengths, respectively; it also suppressed 99% of rice blast severity. In E2, shoot and root dry matter biomass and length, photosynthetic rate, water use efficiency, total soluble sugar, and chloroplastidic pigments were superior in BRS Primavera CL seedlings treated with 2 Si t ha−1 and T. asperellum pool or PGPM mixture. Higher salicilic and jasmonic acid levels were found in seedlings treated with Si and T. asperellum pool, individually. These physiological characteristics may explain, in part, the higher vigor of upland rice seedlings promoted by the synergistic effect between silicate fertilization and beneficial microorganisms.



Ameliorative effect of the anticancer agent salinomycin on cadmium-induced hepatotoxicity and renal dysfunction in mice

Abstract

This study presents experimental data on the effects of the tetraethylammonium salt of salinomycinic acid (Sal) on Cd-induced hepatotoxicity and renal dysfunction in Cd-treated mice compared to those of meso-2,3-dimercaptosuccinic acid (DMSA). Forty 60-day-old male ICR mice were randomized into five groups: control group (untreated mice), Cd group (Cd(II) acetate 20 mg/kg body weight provided orally once per day for 14 days), Cd + DMSA group (exposed to Cd(II) acetate as the Cd-exposed group followed by DMSA 20 mg/kg body weight provided orally once per day for 14 days), and Cd + Sal group (exposed to Cd(II) acetate as the Cd-exposed group followed by Sal 20 mg/kg body weight once per day for 14 days). Cd intoxication of mice induced significant liver and kidney injury and a significant elevation of the concentration of Cd in both organs. Treatment of Cd-exposed mice with DMSA or Sal restored the levels of the renal and hepatic functional markers and significantly decreased the concentration of the toxic metal ion in both organs. Administration of Sal improved Cd-induced alterations of the endogenous levels of the essential metal ions. Histological studies revealed that the antibiotic more effectively ameliorated the Cd effect on the liver morphology compared to DMSA. Taken together, the results confirm that the anticancer agent salinomycin is a promising antidote to Cd poisoning.



Seasonal toxicity variation in light-textured soil amended with urban sewage sludge: interaction effect on cadmium, nickel, and phytotoxicity

Abstract

Sewage sludge is increasingly used as an organic amendment to agricultural soils, especially to soils containing little organic matter. However, little is known on the impact of this biowaste on seasonal changes of nickel and cadmium toxicity in a sandy loam soil. Accordingly, the aim of this field-scale study was to evaluate the seasonal phytotoxicity according to Cd, Ni, and dehydrogenase variation in an agricultural soil during two successive annual amendments with increasing amounts of urban sludge (0, 40, 80, and 120 t ha−1 year−1). Sampling was carried out at the end of dry season (EDS) and at the end of wet season (EWS) during 2 years 2012/2013. Sludge application significantly increased the amount of organic matter and dehydrogenase activity in the soil. In order to explain the seasonal variation of Cd and Ni, pH and electrical conductivity were also monitored in this study. The increased rate of sewage sludge addition slightly reduced the pH but soil remained above neutrality. The electrical conductivity which reflects soil salinity was strongly correlated with Cd and Ni content that increased with sludge dose. Salinity and heavy metals were highest at EDS 2013. In addition, soil phytotoxicity testing was performed by the evaluation of lettuce seed germination for 120 h. Although heavy metal content did not generally exceed Tunisian thresholds (3 and 75 mg kg−1 for Cd and Ni, respectively), the seed germination index decreased with sewage sludge dose at all seasons. In general, we observed a significant effect of seasonal variation for all studied parameters. Sewage sludge reuse could be a feasible way to improve soil organic matter but toxicity risks consistently increased with time.



Deposition, acute toxicity, and bioaccumulation of nickel in some freshwater organisms with best-fit functions modeling

Abstract

Although nickel (Ni) frequently enters into water, documents on speciation of the metal and its toxicity to freshwater organisms are scanty. Laboratory experiments, made in this study, with 1.0–5.0 mg/L of Ni revealed that Ni was not quickly removed from water. Application of cubic regression followed by classical optimization technique showed that maximum reduction time (T) of Ni in water ranged between 60 to 65 h. Ninety-six hours of LC50 value of Ni to crustacean Diaptomus forbesi, fish Cyprinus carpio, and worm Branchiura sowerbyi was respectively 5.43, 14.70, and 19.73 mg/L. Normalizing the lethal values and plotting them against time, it was observed that C. carpio was more sensitive than D. forbesi, which was not reflected in the 96-h LC50 values. However, sensitivity of these organisms to Ni was better explained by power regression equation (M = aNib), which exhibited that mortality (M) of D. forbesi and B. sowerbyi increased slowly between 24 to 72 h, increasing sharply at 96 h, while mortality of C. carpio increased steadily every 24 till 96 h. Experiment with 1.0 mg/L of Ni in outdoor vats showed that crustacean zooplankton and worms accumulated high concentration of Ni, while only gut and liver tissues of fish C. carpio accumulated trace and moderately high concentration of Ni, respectively. It is assumed that toxicity of Ni to fish is mediated primarily through gill.



Di-(2-ethylhexyl)-phthalate induces glucose metabolic disorder in adolescent rats

Abstract

As a plasticizer, di-(2-ethylhexyl)-phthalate (DEHP) is widely added in various commercial products. Some researchers had suggested that DEHP has adverse effects on the glucose metabolism, but the mechanisms remain unclear. Adolescent Wistar rats were divided into four groups and administered DEHP by gavage at 0, 5, 50, and 500 mg kg−1 d−1 for 28 days. ELISA was used to quantify the serum insulin and leptin levels; RT-PCR, immunohistochemistry, and Western blot were used to detect the mRNA and protein expressions of Janus-activated kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), suppressor of cytokine signaling 3 (SOCS3), leptin receptor (Ob-R), and insulin receptor (IR) in liver and pancreas In comparison to the control group, the DEHP-treated rats showed the following: (1) higher organ coefficient of liver; (2) higher fasting blood glucose levels, higher fasting serum insulin and leptin levels, higher insulin resistance index homeostasis model assessment; (3) lower protein levels of Ob-R and IR in the liver and pancreas; (4) higher protein levels of JAK2 and STAT3 in the liver; and (5) higher protein and mRNA levels of SOCS3 in the liver and pancreas. Exposure to DEHP induced glucose metabolic disorder in the adolescent rats, and the mechanism is that DEHP may interfere with the JAK2/STAT3/SOCS3 pathway, regulated the sensitivity of the insulin receptor and leptin receptor.



It started from scratch



Tribute to Reviewers



Issue Information



New Zealand Dermatological Society Inc. Annual Scientific meeting Queenstown, New Zealand August 2017 Convener: Dr Paul JM Salmon



Dermatology Practice Consolidation Fueled by Private Equity Investment

This Viewpoint discusses the consolidation of dermatology practices facilitated by private equity investment and the potential consequences for the specialty and patients.

Insect cuticular melanins are distinctly different from those of mammalian epidermal melanins

Summary

Melanin from several insect samples were isolated and subjected to chemical degradation and HPLC analysis for melanin markers. Quantification of different melanin markers reveals that insect melanins are significantly different from that of the mammalian epidermal melanins. The eumelanin produced in mammals are derived from the oxidative polymerization of both 5,6-dihydroxyindole and 5,6-dihydroxyindole-2-carboxylic acid. The pheomelanin is formed by the oxidative polymerization of cysteinyldopa. Thus dopa is the major precursor for both eumelanin and pheomelanin in mammals. But insect eumelanin appears to be mostly made from 5,6-dihydroxyindole and originate from dopamine. More importantly, our study points out the wide spread occurrence of pheomelanin in many insect species. In addition, cysteinyldopamine and not cysteinyldopa is the major precursor for insect pheomelanin. Thus both eumelanin and pheomelanin in insects differ from higher animals by using dopamine and not dopa as the major precursor.

This article is protected by copyright. All rights reserved.



Phosphorus speciation and release kinetics of swine manure biochar under various pyrolysis temperatures

Abstract

Converting swine manure to biochar is an effective way to recycle valuable nutrients, but there are few reports on its feasibility as a phosphorus (P) source. The objective of this study was to clarify the unique nature, including P speciation, of manure biochar products under various pyrolysis temperatures. We used solution 31P nuclear magnetic resonance and P K-edge X-ray adsorption near-edge spectroscopy (P XANES) to characterize P species in swine manure biochar. For every 100 °C increment starting from 300 °C, the P content in manure biochar increased by 2.16 to 3.37 g kg−1. However, above 400 °C, organic P species did not appear anymore, and only inorganic P, including orthophosphate and pyrophosphate, existed. P K-edge XANES spectra further showed all biochar samples had higher percentages of Ca3 (PO4)2 and NaP2O7, and lower percentages of FePO4, AlPO4, and inositol hexaphosphate compared to manure. Interestingly, percentages of Ca3(PO4)2, FePO4, and AlPO4 in MB400 (indicating manure pyrolysed at 400 °C) were comparable with those in MB700 while the percentage of NaP2O7 was higher in MB400. Phosphorus release from MB400 maintained a relatively high level at 0.33 g kg−1 during the whole 300-h observation period. These results suggest that with a suitable pyrolysis temperature, it was feasible for manure biochar to be a P source alternative.

Graphical abstract



Lighting Van Leeuwenhoek's samples

Abstract
Possible techniques for lighting opaque samples while using Van Leeuwenhoek microscopes have been tested, and the results are presented in relation to published material. The design of the microscope causes the sample to be in shadow with any form of top-lighting. It is therefore suggested that Van Leeuwenhoek's hinted 'particular method of observing' might refer to a different style of microscope as shown in the frontispiece of the sale catalogue for his microscopes, and available at that time for purchase from sellers of optical equipment.

The Editor's Choice



Editorial board



Prefrontal recruitment mitigates risk taking behavior in HIV infected young adults

Abstract
Background
HIV-infected (HIV+) young adults often engage in risk taking behavior. However, the disruptive effects of HIV on the neurobiological underpinnings of risky decision-making are not well understood.
Methods
Risky decision-making, measured via the Iowa Gambling Task (IGT), was compared voxel-wise to resting cerebral blood flow (rCBF) acquired via arterial spin labeling. Separate topographical maps were obtained for HIV uninfected (HIV–; n=62) and HIV+ (n=41) young adults (18-24 years old) and were compared to the full cohort of participants. For the HIV+ group, rCBF was compared to recent and nadir CD4.
Results
IGT performance was supported by rCBF in three distinct brain regions: Region-I, -II, and -III. The relationship between IGT performance and rCBF in HIV+ individuals was most robust in Region-I, ventromedial prefrontal and insular cortices. Region-II contained strong relationships for both HIV– and HIV+. Region-III, dorsolateral prefrontal and posterior cingulate cortices, contained relationships that were strongest for HIV– controls. IGT performance was intact among HIV+ with higher rCBF in either Region-I or Region-III. By contrast, performance was worse among HIV+ individuals with lower rCBF in both Regions-I, and –III when compared to HIV– controls (p=0.01). rCBF in Region-III was reduced in HIV+ compared to HIV– individuals (p=0.04), and positively associated with nadir CD4 (p=0.02).
Conclusions
Recruitment of executive systems (Region-III) mitigates risk taking behavior in HIV+ and HIV– individuals. Recruitment of reward systems (Region-I) mitigates risk taking behavior when Region-III is disrupted due to immunological compromise. Identifying individual recruitment patterns may aid anatomically directed therapeutics or psychosocial interventions.

Reply to Weinbren and Collins



Drying–submergence alternation enhanced crystalline ratio and varied surface properties of iron plaque on rice ( Oryza sativa ) roots

Abstract

Iron plaque (IP) is valuable in nutrient management and contaminant tolerance for rice (Oryza sativa) because it can adsorb various nutrients and toxic ions. Crystalline ratio (CR) can be defined as the proportion of crystalline iron (CI) to total IP to describe IP crystallinity. Although the knowledge on IP has abounded, the information on the relationship among its formation condition, surface properties, and CR remains insufficient. In this study, quartz sand–soil cultivation with rice was conducted to explore the effect of drying–submergence alternation (DSA) on CI, amorphous iron (AI), CR, root oxidizing capacity (ROC), and surface properties of IP with different treatment durations and at different stages. Fourteen-day DSA treatment increased CI to 2.20 times of that after continuous submergence (CS) but decreased AI to 72.3% of that after CS. Correspondingly, CR was raised to 6.89% from 4.08%. Remarkably, CR of IP after DSA ending in submergence and ending in drying was 6.89% and 4.23%, respectively. In addition, ROC after 14-day DSA was enhanced to twice of that after CS. Results from scanning electronic microscope suggested that 14-day DSA induced thinner sheets with finer particles in IP compared to that after CS. Results from X-ray diffraction revealed that IP contained higher proportions of goethite, lepidocrocite, magnetite, and hematite after DSA than those after CS. Variable charge and surface area of IP after DSA were only 26.5% and 32.0% of those after CS, respectively. Together, our results indicated that proper strength DSA promoted ROC and transformation from AI to CI, and consequently increased CR of IP, while it changed its surface properties.



The effect of animal grazing on vegetation and soil and element cycling in nature

Abstract

Appropriate level of bio-components in blood plasma of animals is associated with their concentration in soil and in green fodder. Cycling of elements in nature and their adequate level in animal organisms result in proper functioning of an organism as a whole (Khan et al. 68:279–284, 2007). Therefore, it is important to analyse soil and green fodder for these components. If some deficits are found, it is important to supplement components responsible for e.g. proper growth and development of a young organism, proper reproduction and, consequently, good quality of obtained product, which may affect human health (Minzanova et al. 134:524–533, 2015). The aim of the presented study was to demonstrate the effect of extensive grazing by sheep on vegetation and soil and to analyse the cycling of some elements important for animal health. The effects of vegetation season on the concentration of potassium in soil and differences in the concentration of some elements in soil, green fodder and animal blood were demonstrated.



Patients' preferences for adjuvant sorafenib after resection of renal cell carcinoma in the SORCE trial: what makes it worthwhile?

Abstract
Background
We sought to determine the survival benefits that patients judged sufficient to warrant adjuvant therapy with sorafenib for 1 year, or for 3 years after resection of renal cell carcinoma (RCC) in the SORCE trial.
Methods
SORCE participants from all sites in Australia and New Zealand, and selected sites in the UK, completed a validated preferences questionnaire at months 0, 3, 15 and 42 to elicit the minimum survival benefits they judged sufficient to warrant adjuvant sorafenib for 1 year (versus observation), or for 3 years (versus 1 year). The questionnaires used reference survival times of 5 years and 15 years; and reference survival rates at 5 years of 65% and 85%.
Results
The 233 participants had a median age of 57 years (range 29 to 78) and 71% were male. For 1 year of sorafenib versus no adjuvant therapy, the median benefits in survival times judged sufficient to warrant treatment were an extra 9 months beyond 5 years and an extra 1 year beyond 15 years; the median benefit in survival rates were an extra 4% beyond 65% and an extra 3% beyond 85% at 5 years. For 3 years of sorafenib versus 1 year of sorafenib, the median benefit in survival time judged sufficient to warrant extended treatment was an extra 1 year beyond both 5 years and 15 years. Participants randomly allocated treatment with sorafenib judged larger benefits necessary than those allocated placebo. Participants' preferences were not associated with their baseline characteristics or the interval from randomisation.
Conclusion
Most participants judged an extra year of survival necessary to warrant 1 year of adjuvant sorafenib worthwhile, and an additional year of survival to warrant extending the duration of sorafenib from 1 year to 3 years. Patients' preferences are important in shared-decision-making.SORCE trial clinical trials number = NCT00492258

Removal of lead from aqueous solution using polyacrylonitrile/magnetite nanofibers

Abstract

Lead is known for its toxic and non-biodegradable behavior. The consumption of lead-contaminated water is one of the major threat the world is facing nowadays. In this study, polyacrylonitrile (PAN) and magnetite (Fe3O4) composite nanofiber adsorbent was developed for Pb2+ removal in batch mode. The synthesis was done by a simple and scalable process of electrospinning followed by chemical precipitation of Fe3O4. The nanofibers thus obtained were characterized through FTIR, zeta potential analyzer, and scanning electron microscope (SEM) and were analyzed for their adsorption capability for Pb2+ ions. The amount of metal ion adsorbed was influenced by the initial metal ion concentration, the time the adsorbent was in contact, the amount of nanofiber, and the pH of the solution. The experimental data fitted well with pseudo 2nd-order and Langmuir adsorption isotherm model. The nanofibers showed high adsorption capability and could be recommended for Pb2+ removal successfully.



Vulvar swelling as the first presentation of Crohn's disease in children—A report of three cases

Abstract

Vulvar swelling is a rare manifestation of metastatic Crohn's disease in children. It often predates gastrointestinal symptoms and can be the first presentation of pediatric Crohn's disease. We report three cases of pediatric Crohn's disease presenting with vulvar swelling. We discuss this rare presentation and its treatment and highlight the importance of recognizing it so that prompt investigation and appropriate management can be initiated.



Bleomycin-induced flagellate erythema after venous malformation sclerosis—Case report and brief review

Abstract

Bleomycin-induced flagellate erythema is a rare but typical skin toxicity of bleomycin. We report the case of a boy with a left foot venous malformation who developed this skin rash after two sessions of bleomycin intralesional injection. We discuss the mechanism and characteristics of this reaction to bleomycin, which is usually benign and self-limited. We conclude that, although rare, flagellate pigmentation can occur when bleomycin is used as a sclerosant in children.



Prevalence of sun protection behaviors in Hispanic youth residing in a high ultraviolet light environment

Abstract

Although rates of late-stage melanoma are rising in Hispanics, particularly those living in high ultraviolet light environments, little is known about the prevalence of sun protective behaviors in Hispanic children. We analyzed baseline data including frequency of sunburn, sun protective behaviors, level of U.S. acculturation, and skin phototype from a cross-sectional survey of 2003 Hispanic elementary school children in Los Angeles, California, who participated in a skin cancer prevention intervention. Although the Hispanic children reported frequently engaging in some sun protective behaviors, they also had a high rate of sunburn (59%) that exceeded previous national estimates for non-Hispanic white children (43%). Fewer U.S.-acculturated children reported more frequent shade-seeking at home (P = .02), along with less shade-seeking at school (P = .001) and more sunscreen use at school (P = .02). The surprisingly high rate of sunburn in Hispanic children suggests that the way in which they are practicing sun protection is not preventing sunburns. Sun safety interventions should be targeted toward Hispanic youth to provide them with practical methods of effective sun protection, in addition to education on the risks of high sun exposure.



New soft tissue filler derived from autologous keratin and fibroblast for neck wrinkles

Summary

Objective

To assess the effectiveness of injection of autologous keratin gel and fibroblast for neck aging as soft tissue filler.

Materials and methods

Totally 30 volunteers received treatment of autologous keratin and fibroblast for neck wrinkles and 5 of them received hyaluronic acid (HA) treatment as control. Clinical features of the volunteers were collected at 1st, 3rd, 6th, 12th, and 24th months after treatment. The volunteers were independently assessed using Lemperle's methods at different time points.

Results

The neck wrinkles ameliorated observably and no severe complications were reported after treatment. The effect and maintain time of autologous keratin and fibroblast were better than HA control.

Conclusion

Autologous keratin and fibroblast as soft tissue filler is an effective treatment option for neck rejuvenation with long-term efficacy for reversing of skin aging.



Delayed allergic hypersensitivity to hyaluronidase during the treatment of granulomatous hyaluronic acid reactions

Summary

Hyaluronic acid (HA) is now extensively employed for esthetic concerns. In the majority of cases, HA is considered to be safe, but mild-to-severe complications can occur in a few cases. Hyaluronidase is enzymes that degrade HA, also being suggested for the treatment of HA filler-induced complications. However, hyaluronidase has been reported to be a potential cause of allergic responses in some anesthetic and ophthalmic literature. However, there are few reports of allergic reactions to hyaluronidase in cosmetic medicine. We herein report on a 39-year-old Asian woman who developed a delayed allergic hypersensitivity to hyaluronidase in the treatment process of HA-related granulomatous reaction.



Serum homocysteine levels in acne patients

Summary

Objective

This study was designed to investigate serum homocysteine (HCY) levels in acne patients.

Methods

Acne patients (n = 124) and healthy volunteers (n = 70), matched in terms of both age and sex, were enrolled. Serum HCY levels for all subjects were measured by a clinical laboratory.

Results

Serum HCY levels in male and female patients with severe and moderate acne were significantly higher than in the healthy control group (P < .05). The constituent ratio of male and female acne patients with HCY above the normal range (10 mmol/L) was significantly higher than the healthy control group. The severity of acne patients was positively correlated with serum homocysteine concentration, (P < .01).

Conclusion

Hyperhomocysteinemia may be an independent risk factor for acne vulgaris. Detection of serum HCY is important for acne patients.



Severe Stevens-Johnson syndrome/toxic epidermal necrolysis overlap syndrome—beyond skin involvement

Abstract

Stevens–Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are rare but serious dermatologic diseases with many potential multisystem complications. We describe the case of an 8-year-old girl who developed severe SJS/TEN overlap syndrome (25% of her body surface area was affected) complicated by pancreatitis and bronchiolitis obliterans. These rare complications emphasize the need for careful, intensive monitoring of possible complications and an interdisciplinary team approach to provide optimal treatment and follow-up.



Demographic characteristics of teenage boys with horizontal striae distensae of the lower back

Abstract

Background

This study examines the clinical characteristics and demographics of teenage boys with horizontal striae distensae of the lower back in an outpatient setting.

Methods

Retrospective medical chart reviews and telephone survey studies were completed on an outpatient cohort of 12 boys 11 to 17 years of age with a clinical diagnosis of transverse striae distensae of the lower back at a single-center, university-based, pediatric dermatology practice. We evaluated the clinical features of the striae, participant demographic characteristics, and past medical history. A review of the literature concerning risk factors was conducted using PubMed and Google Scholar.

Results

Of the 14 patients we contacted, 12 agreed to participate. The average age of onset for the striae was 14.3 years. All boys were above the 50th percentile in height at the time of onset. Eight (66.7%) reported a significant growth spurt before the appearance of the stretch marks. Most were asymptomatic. None of the boys had a history of unmonitored exogenous steroid use or prior infection with Bartonella henselae or Borrelia burgdorferi. Only one (8.3%) had a chronic medical condition. Eleven (91.7%) had at least one first-degree relative with striae distensae.

Conclusion

Our results indicate that horizontal striae distensae of the lower back in adolescent boys is associated with a rapid growth spurt, tall stature, and family history of striae distensae. There is no association between this type of striae distensae and any chronic medical condition, bacterial infection, or exogenous steroid use. Thus a careful review of systems and counseling without further medical testing is reasonable management.



Juvenile mycosis fungoides with large-cell transformation: Successful treatment with psoralen with ultraviolet A light, interferon-alfa, and localized radiation

Abstract

Mycosis fungoides with large-cell transformation is historically associated with a poor prognosis. Pediatric cases of mycosis fungoides with large-cell transformation are rare, with only three other cases reported in the literature. We present the first case of a child with almost complete remission of his mycosis fungoides with large-cell transformation shortly after administration of psoralen plus ultraviolet A, interferon-alfa, and localized radiation.