Comparison of high-flow CSF leak closure with nasoseptal flap following endoscopic endonasal approach in adult and pediatric populations

xlomafota13 shared this article with you from Inoreader Comparison of high-flow CSF leak closure with nasoseptal flap following endoscopic endonasal approach in adult and pediatric populations Via leak of cerebrospinal fluid Int Forum Allergy Rhinol. 2021 Oct 30. doi: 10.1002/alr.22910. Online ahead of print. NO ABSTRACT PMID:34719119 | DOI:10.1002/alr.22910 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Can prestin level be a biomarker for determining sensorineural hearing loss?

xlomafota13 shared this article with you from Inoreader Can prestin level be a biomarker for determining sensorineural hearing loss? Via Auris Nasus Larynx Publication date: Available online 31 October 2021 Source: Auris Nasus Larynx Author(s): Sadettin Emre, Turgut Karlidag, Süleyman Aydın, Irfan Kaygusuz, Erol Keles, Abdulvahap Akyigit, Sinasi Yalcin View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Radiation Recall Dermatitis following Radioactive Iodine Therapy: A New Observation

xlomafota13 shared this article with you from Inoreader Radiation Recall Dermatitis following Radioactive Iodine Therapy: A New Observation Via radioactive iodine Case Rep Oncol Med. 2021 Oct 22;2021:8422748. doi: 10.1155/2021/8422748. eCollection 2021. ABSTRACT A 47-year-old female, who had previously received adjuvant right breast radiation for ductal carcinoma in situ, presented with right breast edema, erythema, and pain. This developed about two and a half weeks following radioactive iodine therapy for thyroid carcinoma. A biopsy was performed to rule out malignancy, since inflammatory breast cancer can present with similar symptoms. Th is confirmed radiation recall dermatitis (RRD) as the most likely diagnosis. RRD is an inflammatory reaction occurring in a previously irradiated field and was first described in 1959. Subsequent reports in the literature have associated it with the administration of other drugs, mostly chemotherapy. To our knowledge, this is the first reported case of RRD following radioactive iodine therapy. PMID:34721913 | PMC:PMC8556093 | DOI:10.1155/2021/8422748 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Low-activity 125I implantation into VX2 tumor rabbits and quantitative evaluation of the precise therapeutic effect

xlomafota13 shared this article with you from Inoreader Low-activity <sup>125</sup>I implantation into VX<sub>2</sub> tumor rabbits and quantitative evaluation of the precise therapeutic effect Via radioactive iodine Exp Ther Med. 2021 Dec;22(6):1438. doi: 10.3892/etm.2021.10873. Epub 2021 Oct 12. ABSTRACT There is still controversy about quantitatively evaluating the therapeutic effect of radioactive low-activity iodine-125 seeds (125I seeds). In the present study, a paired VX2 tumor model in a rabbit hind leg muscle was established, which is virus-induced anaplastic squamous cell carcinoma characterized by hypervascularity, rapid growth and easy propagation in the skeletal muscle. 125I seeds with 0.4 and 0.7 mCi activity were implanted into the left and right legs, respectively, using a radiation treatment planning system under positron emission tomography (PET)/computed tomography (CT) guidance. PET/CT scans and hematoxylin and eosin staining were observed at 72 h and 2 and 4 weeks after implantation to assess the therapeutic effect. The results showed that the average tumor length and standard uptake value (SUV) decreased over time, and both 125I seed groups achieved therapeutic effects at 4 weeks post-implantation. Quantitative evaluation of tumor inhibition rate, SUV variation and tumor marker ratio (Bcl-2/Bax) suggested that 0.7 mCi 125I seeds were more suitable than 0.4 mCi seeds in a clinical setting. PMID:34721680 | PMC:PMC8549107 | DOI:10.3892/etm.2021.10873 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

3D-4K exoscope-assisted temporal bone dissection: a new frontier in surgical training

xlomafota13 shared this article with you from Inoreader 3D-4K exoscope-assisted temporal bone dissection: a new frontier in surgical training Via http://link.springer.com/journal/405,European archives of oto-rhino-laryngology,The European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino Eur Arch Otorhinolaryngol. 2021 Oct 31. doi: 10.1007/s00405-021-07137-1. Online ahead of print. ABSTRACT PURPOSE: To assess if 3D-4K exoscope is a valuable tool for temporal bone dissection and to evaluate its teaching potential. METHODS: Six consecutive 3D-4K-exoscope-assisted cortical mastoidectomies were performed by a novice, an intermediate and an expert surgeon (two dissections each). All dissections were entirely recorded and later evaluated independently by three other experienced surgeons. The dissection end-product was evaluated according to the Melbourne Mastoidectomy Scale (MMS). Paired t test was used to assess whether novice and intermediate surgeons have a score improvement in the second dissection compared to the first one. Surgeons' interactions, depth effect, and 3D impression were also assessed to perform a subjective analysis. RESULTS: Mean MMS scores for the novice, intermediate and expert surgeon were 11.3 ± 2.8, 13.8 ± 3.9 and 19 ± 1.3, respectively. Paired t test demonstrated a statically significant improvement between the first and the second dissection both for the novice and the intermediate surgeon (+ 4.7 and + 7 points; p = 0.0002). A high-quality magnification of the temporal bone was obtained, allowing the expert surgeon to identify all the anatomical structures without injuring them. The exoscope was capable of providing a high involvement in the dissections with very effective interactions between the expert surgeon and the trainees, that had access to the same surgical field view. CONCLUSION: 3D-4K-exoscope resulted adequate for a safe and effective mastoidectomy and showed a high potential for training and educational purposes. It can represent a valid option for surgical training of temporal bone dissection and a new interactive tool to understand the complex temporal bone anatomy. PMID:34719728 | DOI:10.1007/s00405-021-07137-1 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

MicroRNA-424 alleviates neurocyte injury by targeting PDCD4 in a cellular model of cerebral ischemic stroke

xlomafota13 shared this article with you from Inoreader MicroRNA-424 alleviates neurocyte injury by targeting PDCD4 in a cellular model of cerebral ischemic stroke Via Experimental and therapeutic medicine Exp Ther Med. 2021 Dec;22(6):1453. doi: 10.3892/etm.2021.10888. Epub 2021 Oct 15. ABSTRACT Cerebral ischemic stroke is the primary cause of stroke-associated mortality and disability, and current therapeutic options are limited and ineffective. The present study aimed to investigate the potential of apoptotic therapy and the role of microRNA (miR)-424 in cerebral ischemic stroke. PC12 cells, a cloned cell line from rat adrenal pheochromocytoma, were treated with CoCl2 to construct a cellular ischemia model. mRNA and protein levels of programmed cell death protein 4 (PDCD4), Bcl-2, Bax, caspase-3, PI3K and AKT were evaluated using reverse transcription-quantitative PCR and western blot analyses, respectively. Cell Counting Kit-8 assays were performed to examine cell viability in the ischemia model. Flow cytometry was conducted to evaluate the apoptosis of ischemic cells. Furthermore, a luciferase assay was performed to verify the target gene of miR-424. It was revealed that the expression level of miR-424 was downregulated in the ischemia model, while the expression of PDCD4 was upregulated. Moreover, the expression of miR-424 was increased after treatment with miR-424 mimics. The mRNA and protein expression of PDCD4 was upregulated after transfection with pcDNA3.1-PDCD4. PDCD4 was predicted and demonstrated to be a target of miR-424. Notably, overexpression of miR-424 increased cell viability and inhibited apoptosis in the ischemia model, which was reversed by co-treatment with pcDNA3.1-PDCD4. Furthermore, overexpression of miR-424 regulated the expression of PDCD4, Bax, Bcl-2, phosphorylated-PI3K/AKT and caspase-3, which was restored after co-transfection with pcDNA3.1-PDCD4. Collectively, the results indicated that miR-424 regulated the progression of cerebral ischemic stroke in a cellular model by targeting PDCD4. PMID:34721695 | PMC:PMC8549098 | DOI:10.3892/etm.2021.10888 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Pueratin improves diminished ovarian reserve by inhibiting apoptosis

xlomafota13 shared this article with you from Inoreader Pueratin improves diminished ovarian reserve by inhibiting apoptosis Via Experimental and therapeutic medicine Exp Ther Med. 2021 Dec;22(6):1423. doi: 10.3892/etm.2021.10858. Epub 2021 Oct 11. ABSTRACT Pueratin (Pue) is an extract from Pueraria lobata, and exhibits therapeutic effects for the treatment of inflammation. However, the beneficial effects and mechanisms underlying Pue in the treatment of diminished ovarian reserve (DOR) remains to be fully elucidated. The aim of the present study was to investigate the effect of Pue on Bcl-2 and Bax protein expression in rats with DOR, associated with infertility within clinical practice, induced by 4-vinylcyclohexene diepoxide (VCD). A model of DOR was established in female Sprague Dawley rats by an intraperitoneal injection of 80 mg/kg VCD daily for 45 days. From day 1, the Sprague Dawley rats were orally administered with drugs daily for 45 days. They were divided into normal, model, Pue-low dose (L), Pue-medium dose (M) and Pue-high dose (H) groups (50, 100 and 300 mg/kg Pue, respectively). Foll icle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) levels were subsequently detected using ELISA. H&E staining and TUNEL staining were used to evaluate histopathological changes and apoptosis levels in the ovary, respectively. Bcl-2 and Bax protein expression levels in rat ovaries were evaluated using immunohistochemistry and western blotting. Compared with those in the model group, FSH and LH levels in the Pue-L, -M and -H groups were significantly decreased, whilst E2 levels were significantly increased (P<0.05). After intragastric administration, the volume of the ovaries and uteri of rats in the Pue groups was increased compared with the model group, and the numbers of primordial follicles and primary follicles were also increased. The number of apoptotic cells and the expression of Bax were significantly reduced in a dose-dependent manner (P<0.05), compared with the model group. In addition, Bcl-2 protein expression and t he Bcl-2/Bax ratio were found to be significantly increased in the Pue-treated groups in a dose-dependent manner (P<0.05), compared with the model group. In conclusion, Pue treatment improved ovarian function by regulating hormone balance in addition to Bcl-2 and Bax expression. PMID:34721677 | PMC:PMC8549093 | DOI:10.3892/etm.2021.10858 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

MicroRNA-98-5p inhibits human mesangial cell proliferation and TNF-α and IL-6 secretion by targeting BTB and CNC homology 1

xlomafota13 shared this article with you from Inoreader MicroRNA-98-5p inhibits human mesangial cell proliferation and TNF-α and IL-6 secretion by targeting BTB and CNC homology 1 Via Experimental and therapeutic medicine Exp Ther Med. 2021 Dec;22(6):1436. doi: 10.3892/etm.2021.10871. Epub 2021 Oct 12. ABSTRACT MicroRNA (miR)-98-5p has been reported to be involved in the development of lupus nephritis (LN); however, its specific role in LN remains unclear. The present study aimed to investigate the effect of miR-98-5p on human mesangial cell proliferation and the secretion of TNF-α and IL-6. Reverse transcription-quantitative PCR (RT-qPCR) and western blotting were used to analyze the level of gene and protein expression, respectively. Cellular proliferation was assessed using a Cell Counting Kit-8 (CCK-8) assay. ELISA was used to detect the secretion of TNF-α and IL-6 by human mesangial cells. RT-qPCR analysis revealed that miR-98-5p expression was downregulated in LN renal tissues compared with control renal tissues. Overexpression of miR-98-5p inhibited human mesangial cell proliferation and the secretion of TNF-α and IL-6, whereas miR-98-5p-knock down demonstrated the opposite effect. Dual luciferase reporter assays demonstrated that miR-98-5p directly targeted BTB and CNC homology 1 (BACH1). BACH1-overexpression promoted human mesangial cell proliferation and the secretion of TNF-α and IL-6, whereas BACH1-knockdown demonstrated the opposite effect. Notably, co-transfection with miR-98-5p mimic inhibited BACH1-overexpression induced human mesangial cell proliferation and the secretion of TNF-α and IL-6. The results of the present study indicated that miR-98-5p inhibited human mesangial cell proliferation and the secretion of TNF-α and IL-6 by targeting BACH1. Therefore, miR-98-5p and BACH1 may represent potential therapeutic targets for LN. PMID:34721678 | PMC:PMC8549099 | DOI:10.3892/etm.2021.10871 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Low-activity 125I implantation into VX2 tumor rabbits and quantitative evaluation of the precise therapeutic effect

xlomafota13 shared this article with you from Inoreader Low-activity <sup>125</sup>I implantation into VX<sub>2</sub> tumor rabbits and quantitative evaluation of the precise therapeutic effect Via Experimental and therapeutic medicine Exp Ther Med. 2021 Dec;22(6):1438. doi: 10.3892/etm.2021.10873. Epub 2021 Oct 12. ABSTRACT There is still controversy about quantitatively evaluating the therapeutic effect of radioactive low-activity iodine-125 seeds (125I seeds). In the present study, a paired VX2 tumor model in a rabbit hind leg muscle was established, which is virus-induced anaplastic squamous cell carcinoma characterized by hypervascularity, rapid growth and easy propagation in the skeletal muscle. 125I seeds with 0.4 and 0.7 mCi activity were implanted into the left and right legs, respectively, using a radiation treatment planning system under positron emission tomography (PET)/computed tomography (CT) guidance. PET/CT scans and hematoxylin and eosin staining were observed at 72 h and 2 and 4 weeks after implantation to assess the therapeutic effect. The results showed that the average tumor length and standard uptake valu e (SUV) decreased over time, and both 125I seed groups achieved therapeutic effects at 4 weeks post-implantation. Quantitative evaluation of tumor inhibition rate, SUV variation and tumor marker ratio (Bcl-2/Bax) suggested that 0.7 mCi 125I seeds were more suitable than 0.4 mCi seeds in a clinical setting. PMID:34721680 | PMC:PMC8549107 | DOI:10 .3892/etm.2021.10873 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Huoxue Qianyang Qutan recipe attenuates Ang II-induced cardiomyocyte hypertrophy by regulating reactive oxygen species production

xlomafota13 shared this article with you from Inoreader Huoxue Qianyang Qutan recipe attenuates Ang II-induced cardiomyocyte hypertrophy by regulating reactive oxygen species production Via Experimental and therapeutic medicine Exp Ther Med. 2021 Dec;22(6):1446. doi: 10.3892/etm.2021.10881. Epub 2021 Oct 14. ABSTRACT Continuous and irreversible cardiac hypertrophy can induce cardiac maladaptation and cardiac remodeling, resulting in increased risk of developing cardiovascular diseases. The present study was conducted to investigate the therapeutic effect of Huoxue Qianyang Qutan recipe (HQQR) on angiotensin II (Ang II)-induced cardiomyocyte hypertrophy. Primary cardiomyocytes were isolated from the cardiac tissue of neonatal rats, followed by flow cytometry detection to confirm the proportion of primary cardiomyocytes. Cell Counting Kit-8 assay and immunofluorescence detection were performed to examine the effect of Ang II and HQQR on cardiomyocyte hypertrophy. Reactive oxygen species (ROS) and a series of metabolic indicators were quantified to investigate the effect of HQQR on Ang II-induced cardiomyocyte hypertrophy. Mitochondrial electron transport chain complex activity and related coding gene expression were determined to explore the effect of HQQR on mitochondrial function. HQQR significantly inhibited Ang II-induced cardiomyocyte hypertrophy and restored Ang II-induced ROS accumulation, metabolic indicators, and membrane potential levels. HQQR also regulated the mitochondrial function related to the sirtuin 1 pathway in Ang II-induced cardiomyocytes by increasing the activity of the mitochondrial electron transport chain complex and affecting the expression of genes encoding mitochondrial electron transport chain complex subunits. HQQR could alleviate Ang II-induced cardiomyocyte hypertrophy by modulating oxidative stress, accumulating ROS and increasing mitochondrial electron transport chain activity. PMID:34721688 | PMC:PMC8549094 | DOI:10.3892/etm.2021.10881 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Low-doses of aspirin promote the growth of human PC-9 lung cancer cells through activation of the MAPK family

xlomafota13 shared this article with you from Inoreader Low-doses of aspirin promote the growth of human PC-9 lung cancer cells through activation of the MAPK family Via Experimental and therapeutic medicine Exp Ther Med. 2021 Dec;22(6):1440. doi: 10.3892/etm.2021.10875. Epub 2021 Oct 12. ABSTRACT Aspirin has been reported for its anti-tumor activity, however, there are few studies on its effects in lung cancer. The present study found that aspirin had a dual role in the proliferation of human lung cancer PC-9 (formerly known as PC-14) and A549 cells, and in human colon cancer HCT116 cells. The cells were treated with 0, 1, 2, 4, 8 and 16 mM aspirin for 24-72 h or 7-12 days and cell proliferation was examined by MTT and colony formation assay. In order to explore the relationship between the proliferation-enhancing effect of low-dose aspirin and mitogen-activated protein kinase (MAPK) signaling activation, PC-9 cells were pretreated with 10 µM PD98059 (a specific inhibitor of ERK), SB203580 (a specific inhibitor of p38) and SP600125 (a specific inhibitor of JNK) for 30 min respectively. Western blot assay was performed to detect the activ ation of MAPK members in PC-9 cells. Cellular apoptosis was detected using flow cytometer-based Annexin V/propidium iodide dual staining. An assessment of MAPK inhibitors was performed to further validate the role of JNK, p38 and ERK in aspirin-promoted PC-9 cell growth. It was demonstrated that aspirin could promote the growth of human PC-9 lung cancer cells and induced MAPK activation at low concentrations. All the MAPK inhibitors tested (PD98059, SB203580 and SP600125) were able to inhibit the aspirin-induced proliferation of PC-9 cells. PMID:34721682 | PMC:PMC8549104 | DOI:10.3892/etm.2021.10875 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.