Toxicity evaluation of 4,4′-di-CDPS and 4,4′-di-CDE on green algae Scenedesmus obliquus : growth inhibition, change in pigment content, and oxidative stress

Abstract

Polychlorinated diphenyl sulfides and polychlorinated diphenyl ethers are two types of dioxin-like pollutants, which are prevalent in aquatic environments. However, to date, limited information is available regarding their toxicity to green algae. In this study, growth inhibition, effect on pigment content, and oxidative stress potentials of 4,4′-di-CDPS and 4,4′-di-CDE on green algae Scenedesmus obliquus were investigated. The results indicate that the EC₅₀ values of 4,4′-di-CDPS after 24, 48, 72, and 96 h of exposure were 1.736, 1.172, 0.994, and 0.820 mg/L, while the corresponding values for 4,4′-di-CDE were 0.697, 1.087, 0.833, and 0.327 mg/L. As compared to the control group, most of the measured pigment content in algal cells significantly decreased after 96-h exposure to these two chemicals, suggesting their suppressive capability on the photosynthesis process in algal cells. Additionally, oxidative stress occurred as demonstrated by the significantly inhibited activities of the antioxidant enzymes (total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GPx)), and high increases in malondialdehyde (MDA) content in all 4,4′-di-CDE-treated groups and some moderate-dose and high-dose treatments with 4,4′-di-CDPS. Acute toxicity tests and biochemical analysis showed that 4,4′-di-CDE was more toxic than 4,4′-di-CDPS on S. obliquus.