DNA methylation-mediated down-regulation of TMEM130 promotes cell migration in breast cancer

xlomafota13 shared this article with you from Inoreader DNA methylation-mediated down-regulation of TMEM130 promotes cell migration in breast cancer Via histochem Acta Histochem. 2021 Nov 8;123(8):151814. doi: 10.1016/j.acthis.2021.151814. Online ahead of print. ABSTRACT BACKGROUND: Breast cancer is the most common female cancer worldwide. DNA methylation is a common modification in epigenetics and affects the prognosis of breast cancer by changing gene expression. In the present study, we aim to investigate the role of DNA methylation in TMEM130 gene expression, and the function of TMEM130 in breast cancer cell migration. METHO DS: The transcriptional expression of TMEM130 was detected by qRT-PCR in breast cancer cell lines and tissues. Bisulfite sequencing PCR (BSP) was used to confirm the methylation status of TMEM130 promoter. Then, TMEM130 was transfected in breast cancer cell lines and to explore its role in cell migration by Transwell and western blot. RESULTS: TMEM130 mRNA expression was decreased in breast cancer cell lines and tissues, and consistent with the data in The Cancer Genome Atlas (TCGA). The promoter of TMEM130 was hypermethylated in breast cancer and the expression of TMEM130 could be restored by the methyltransferase inhibitor. Overexpression of TMEM130 could inhibit cell migration ability in breast cancer cell lines. CONCLUSION: Taken together, these results indicate TMEM130 downregulation and hypermethylation might contribute to breast cancer migration and TMEM130 might be a promising biomarker for breast cancer. PMID:34763116 | DOI:10.1016/j.acthis.2021.151814 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.