Anti-inflammatory activities of Gardenia jasminoides extracts in retinal pigment epithelial cells and zebrafish embryos

xlomafota13 shared this article with you from Inoreader Anti-inflammatory activities of <em>Gardenia jasminoides</em> extracts in retinal pigment epithelial cells and zebrafish embryos Via Experimental and therapeutic medicine Exp Ther Med. 2021 Jul;22(1):700. doi: 10.3892/etm.2021.10132. Epub 2021 May 2. ABSTRACT Age-related macular degeneration (AMD) is the most common cause of visual impairment in developed countries. Inflammation serves a critical role in the pathogenesis of AMD. Gardenia jasminoides is found in several regions of China and is traditionally used as an organic yellow dye but has also been widely used as a therapeutic agent in numerous diseases, including inflammation, depression, hepatic and vascular disorders, which may reflect the variability of functional compounds that are present in Gardenia jasminoides extracts (GJE). To investigate the therapeutic potential of GJE for AMD, ARPE-19 cells were treated with lipopolysaccharide (LPS) or LPS plus GJE. GJE significantly decreased LPS-induced expression of proinflammatory cytokines, including IL-1β, IL-6 and TNF-α. In the in vivo study, GJE inhibited CuSO4-induced mi gration of primitive macrophages to the lateral line in zebrafish embryos. GJE also attenuated expression of cytokines (IL-1β, IL-6 and TNF-α), NFKB activating protein (nkap) and TLR4 in ARPE-19 cells. The results of the present study demonstrated the anti-inflammatory potential of GJE in vitro and in vivo, and suggested GJE as a therapeutic candidate for AMD. PMID:34007309 | PMC:PMC8120509 | DOI:10.3892/etm.2021.10132 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.