Therapeutic effect of concentrated growth factor preparation on skin photoaging in a mouse model

a.sfakia shared this article with you from Inoreader Therapeutic effect of concentrated growth factor preparation on skin photoaging in a mouse model Via SAGE Publications Ltd: Journal of International Medical Research: Table of Contents Journal of International Medical Research, Volume 48, Issue 10, October 2020. ObjectiveTo establish a nude mouse model of photoaging and study the therapeutic effect of a concentrated growth factor preparation (CGF) on skin photoaging.MethodsCGF was prepared from blood from Sprague–Dawley rats. A skin photoaging nude mouse model was developed using UV irradiation combined with the photosensitizer, 8-methoxypsoralen. Mice were divided randomly into seven groups (n = 6 per group): normal control, photoaging, mock treatment, saline treatment, CGF treatment, Filoca 135HA treatment, and plasma skin regeneration system irradiation (the latter two were positive controls). Body weight and skin appearance were observed and pathological changes were determined by hematoxylin and eosin staining. Fiber elasticity was evaluated by Weigert staining. Expr ession levels of proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase 1 (MMP1) were determined by immunohistochemistry.ResultsA mouse model with typical features of photoaging skin was successfully developed. CGF significantly improved the skin appearance, wrinkle scores, pathological changes, and fiber elasticity, and increased PCNA and decreased MMP1 expression levels in photoaging mice, comparable to the two positive controls.ConclusionCGF can improve the symptoms of skin photoaging in mice, suggesting that it may have applications in the treatment of skin aging in humans. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.