Reconstituting human cutaneous regeneration in humanized mice under endothelial cell therapy

Much of our understanding of human biology and the function of mammalian cells in tissue regeneration have been derived from mechanistically and genetically manipulated rodent models. However, current models examining epidermal wound repair fail to simultaneously address both the cross-species mechanistic and immunogenic differences. Herein, we describe a multifaceted approach intended to better recapitulate human skin recovery in rodent models. First, immunodeficient NSG mice were intravenously inoculated with human hematopoietic stem cells to become in essence, humanized, and capable of initiating an adaptive immune response.