Insight into the impact of Fe 3 O 4 nanoparticles on anammox process of subsurface-flow constructed wetlands under long-term exposure

Abstract

The increasing use of Fe₃O₄ nanoparticles (NPs) had posed an emerging challenge to wastewater treatment processes, and their potential impact on anaerobic ammonium oxidation (anammox) process of unplanted subsurface-flow constructed wetlands (USFCWs) was investigated firstly under the long-term exposure of different Fe₃O₄ NP concentrations. It was found that Fe₃O₄ NP exposure could improve total nitrogen (TN) removal. The abundance of Candidatus Anammoxoglobus increased significantly at 10 mg/L Fe₃O₄ NPs, while decreased under 1 mg/L Fe₃O₄ NP exposure. Desulfosporosinus and Exiguobacterium increased to some extent at 1 mg/L Fe₃O₄ NPs, suggesting that Fe-anammox played an important role in TN removal. The ROS production increased with the increase of Fe₃O₄ NP concentration, and the integrity of cell membrane was good under Fe₃O₄ NP exposure. The functional genes that related to inorganic ion transport and metabolism and lipid transport and metabolism were upregulated, and cell motility decreased after long-term exposure of 1 mg/L Fe₃O₄ NPs.

Graphical abstract

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