Fluorescence quenching of MoS 2 nanosheets/DNA/silicon dot nanoassembly: effective and rapid detection of Hg 2+ ions in aqueous solution

Abstract

Mercury (Hg) contamination of aquatic sites represents a serious risk for human health and the environment. Therefore, effective and rapid monitoring of Hg in aqueous samples is a challenge of timely importance nowadays. In the present study, a rapid and sensitive mercury sensor based on the fluorescence quenching of MoS₂ nanosheets/DNA/silicon dot nanoassembly has been developed for the efficient detection of mercury(II) in aquatic environments. In this process, silicon dots were synthesized through one-step high-temperature calcinations and thermomagnesium reduction method at 900 °C using rice husk as a silicon source, which demonstrates superior photophysical properties and excitation-dependent fluorescence behavior. The interaction between MoS₂ nanosheets/DNA/silicon dot nanoassembly and Hg²⁺ ions was studied using photoluminescence spectroscopy. The addition of Hg²⁺ ions to the assay solution induced the detachment of fluorescent probe from the surface of MoS₂ nanosheets. Thus, the fluorescent probes sustained its fluorescence intensity. The developed sensor was tested on various concentrations of Hg²⁺ ions ranging from 0 to 1000 nM as well as on various metal ions. In addition, MoS₂ nanosheets/DNA/silicon dot nanoassembly fluorescent Hg sensor efficiently detected the presence of Hg²⁺ ions in real-time water samples, which was comparably detected by the conventional atomic absorbance spectrometer (AAS). Overall, our results highlighted the high reliability of the present approach for environmental monitoring of Hg²⁺ ions, if compared to that of the customary method with a lowest detection limit of 0.86 nM.