Imperative roles of halotolerant plant growth-promoting rhizobacteria and kinetin in improving salt tolerance and growth of black gram ( Phaseolus mungo )

Abstract

The salinity stress causes a major threat for plant growth, yield, and biomass production. The present study was designed to assess the effect of exogenously applied kinetin and halotolerant plant growth-promoting rhizobacteria (H-PGPR) on alleviation of salt stress in black gram (Phaseolus mungo). A total of 15 rhizobacterial isolates obtained from a salt-affected area were analyzed for their capability to improve growth of P. mungo plants growing in greenhouse conditions. Out of the tested rhizobacteria, the two bacterial isolates which exhibited maximum growth potential were screened and their growth-promoting attributes were evaluated. The role of screened H-PGPR and/or kinetin (8 and 10 μM) was evaluated in P. mungo plants irrigated with three levels of brackish water (S1 = 3, S2 = 5, and S3 = 7 dSm⁻¹) under field condition. Salt stress reduced transpiration rate, stomatal conductance, salt tolerance index, growth, leaf area, photosynthetic pigments, leaf relative water content (LRWC), biomass production, and seed yield in subjected plants. Conversely, the salinized plants treated with kinetin and/or H-PGPR exhibited improved levels of chlorophyll contents, LRWC, root growth, shoot growth, biomass production, and seed yield. The H-PGPR and/or kinetin supplementation also reduced electrolyte leakage in salt-stressed plants. Overall, the present findings will be of great value to recognize the mechanism of salt stress alleviation in P. mungo plants under the influence of H-PGPR and/or kinetin.