Inhibition of autophagy promotes human RSV NS1-induced inflammation and apoptosis in vitro

xlomafota13 shared this article with you from Inoreader Inhibition of autophagy promotes human RSV NS1-induced inflammation and apoptosis <em>in vitro</em> Via Experimental and therapeutic medicine Exp Ther Med. 2021 Oct;22(4):1054. doi: 10.3892/etm.2021.10488. Epub 2021 Jul 23. ABSTRACT Human respiratory syncytial virus (RSV) is a major health challenge due to the lack of a safe and effective vaccine and antiviral drugs. RSV non-structural protein 1 (NS1) is the main inhibitor of antiviral signaling pathways in RSV infection; however, the underlying mechanism is unclear. The aim of the present study was to investigate of the role of NS1 and its relationship with autophagy. NS1-Flag plasmid was transfected into A549 cells and the levels of inflammatory cytokines, autophagy markers and apoptosis were detected. In addition, the cells were treated with an autophagy inhibitor, 3-methyladenine for 12 h prior to transfection with the NS1 plasmid to explore the role of autophagy in NS1-transfected cells. The results showed that the production of inflammatory cytokines and autophagy was induced in NS1-transfected cells, and indicated tha t autophagy prevents the production of cytokines and the activation of apoptosis. Furthermore, the results demonstrated that NS1 activated autophagy partly through the mTOR-p70 S6 kinase signaling pathway. The results suggest that autophagy induced by NS1 transfection through the mTOR pathway can hinder the production of inflammatory cytokines and interferon-α and inhibit cell apoptosis, which may help to explain why autophagy has been shown to be beneficial to viral replication in most studies. PMID:34434268 | PMC:PMC8353648 | DOI:10.3892/etm.2021.10488 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.