microRNA-23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12

xlomafota13 shared this article with you from Inoreader microRNA-23 inhibits inflammation to alleviate rheumatoid arthritis via regulating CXCL12 Via Experimental and therapeutic medicine Exp Ther Med. 2021 May;21(5):459. doi: 10.3892/etm.2021.9890. Epub 2021 Mar 3. ABSTRACT Rheumatoid arthritis (RA) is a common systemic, inflammatory and autoimmune disorder. MicroRNAs (miRs) are strongly associated with the initiation and progression of RA. However, the functions and mechanisms underlying miR-23 in RA are not completely understood. Therefore, the present study aimed to investigate the molecular mechanisms underlying miR-23 in RA. A bioinformatics tool (StarBase) and a wide range of experimental assays, including reverse transcription-quantitative PCR, western blotting, luciferase reporter assays and ELISAs, were performed to investigate the biological role of miR-23 in RA. The results indicated that miR-23 was downregulated and chemokine C-X-C motif ligand 12 (CXCL12) was upregulated in RA samples compared with healthy samples. Furthermore, miR-23 overexpression suppressed inflammation via reducing TNF-α, IL-1β and I L-8 expression levels compared with the NC mimic group. Regarding the underlying mechanism, compared with NC mimic, miR-23 mimic decreased CXCL12 mRNA expression by binding to its 3'-untranslated region. Additionally, CXCL12 overexpression reversed miR-23 mimic-mediated effects on inflammation. NF-κB signaling is associated with inflammation. Therefore, the present study indicated that CXCL12 promoted inflammation by activating NF-κB signaling. In conclusion, miR-23 inhibited inflammation to alleviate RA by regulating CXCL12 via the NF-κB signaling pathway, which may serve as a potential target for the diagnosis and treatment of RA. PMID:33777193 | PMC:PMC7967800 | DOI:10.3892/etm.2021.9890 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.