Photodynamic inactivation of Enterococcus faecalis by non-peripherally substituted magnesium phthalocyanines entrapped in lipid vesicles

Publication date: Available online 12 September 2018

Source: Journal of Photochemistry and Photobiology B: Biology

Author(s): Lukasz Sobotta, Jolanta Dlugaszewska, Mateusz Gierszewski, Adam Tillo, Marek Sikorski, Ewa Tykarska, Jadwiga Mielcarek, Tomasz Goslinski

Abstract

Photophysical properties and photodynamic antibacterial potential of magnesium phthalocyanines bearing 2-propoxy, benzyloxy, 3,5-bis(benzyloxy)benzyloxy substituents at non-peripheral positions were studied. The UV–Vis absorption spectra of researched phthalocyanine derivatives were found typical. Extension of peripheral substituent size from 2-propoxy to benzyloxy and finally 3,5-bis(benzyloxy)benzyloxy was accompanied by the rise of quantum yield of fluorescence up to 0.17 and 0.04 in DMF and DMSO, respectively. Similarly, the expansion of the phthalocyanine periphery from the 2-propoxy to benzyloxy and 3,5-bis(benzyloxy)benzyloxy groups resulted in a detectable increase of the singlet oxygen quantum yield values to 0.04, 0.12, 0.14 respectively, which was assessed following direct method of singlet oxygen phosphorescence measurement at 1270 nm. Studied phthalocyanines undergo photobleaching process with the quantum yields at the level of 10⁻⁶ in DMSO and 10⁻⁵ in DMF. The size of phthalocyanine impacted the process of liposomal formulation. Small liposome vesicles containing non-peripherally substituted phthalocyanines with 2-propoxy and benzyloxy substituents were obtained following extrusion method. The unification process of the liposomes loaded with 3,5-bis(benzyloxy)benzyloxy non-peripherally substituted phthalocyanines was not possible. In in vitro antimicrobial photodynamic inactivation study, log reduction values of bacterial (Enterococcus faecalis) growth at 3.61 and 2.99 were achieved for liposomal formulations containing phthalocyanines with 2-propoxy and benzyloxy substituents respectively, whereas phthalocyanine with 3,5-bis(benzyloxy)benzyloxy substituents was inactive. Phthalocyanine with 2-propoxy substituents exhibited relatively low toxicity in Vibrio fischeri bioluminescence test, whereas phthalocyanine with benzyloxy substituents revealed intense bioluminescence, which could be associated with hormesis phenomenon.

Graphical Abstract

Photophysical properties and photodynamic potential of magnesium phthalocyanines bearing 2-propoxy, benzyloxy, 3,5-bis(benzyloxy)benzyloxy at non-peripheral positions was studied. In in vitro antimicrobial photodynamic inactivation study, log reductions of bacterial (Enterococcus faecalis) growth at 3.61, 2.99 were obtained for liposomal formulations containing non-peripherally substituted 2-propoxy and benzyloxy phthalocyanines, respectively.