Morphological and genetical changes of endothelial progenitor cells after in-vitro conversion into photoreceptors

Publication date: Available online 6 April 2018
Source:Journal of Photochemistry and Photobiology B: Biology
Author(s): Shi Qiang, Hiba Amer Alsaeedi, Cheng Yuhong, Hao Yang, Li Tong, Suresh Kumar, Akon Higuchi, Abdullah A. Alarfaj, Rusheni Munisvaradass, Mok Pooi Ling, Pei Cheng
BackgroundRetinal degeneration is a condition ensued by various ocular disorders such as artery occlusion, diabetic retinopathy, retrolental fibroplasia and retinitis pigmentosa which cause abnormal loss of photoreceptor cells and lead to eventual vision impairment. No efficient treatment has yet been found, however, the use of stem cell therapy such as bone marrow and embryonic stem cells has opened a new treatment modality for retinal degenerative diseases. The major goal of this study is to analyze the potential of endothelial progenitor cells derived from bone marrow to differentiate into retinal neural cells for regenerative medicine purposes.MethodsIn this study, endothelial progenitor cells were induced in-vitro with photoreceptor growth factor (taurine) for 21 days. Subsequently, the morphology and gene expression of CRX and RHO of the photoreceptors-induced EPCs were examined through immunostaining assay.FindingsThe results indicated that the induced endothelial progenitor cells demonstrated positive gene expression of CRX and RHO. Our findings suggested that EPC cells may have a high advantage in cell replacement therapy for treating eye disease, in addition to other neural diseases, and may be a suitable cell source in regenerative medicine for eye disorders.