Iris pigmented lesions as a marker of cutaneous melanoma risk: an Australian case-control study

Abstract

Background

Iris naevi and iris freckles have a frequency of 4% and 50% in the European population. They are associated with dysplastic naevi, but few studies examine their link to cutaneous melanoma.

Objectives

To assess whether iris pigmented lesions are a predictive indicator for cutaneous melanoma.

Methods

This is a melanoma case-control study of 1254 European-background Australians. Sun exposure and melanoma history, a saliva sample for DNA analysis, and eye photographs taken with a digital camera were collected from 1117 participants. Iris images were assessed by up to four trained observers for the number of iris pigmented lesions. The data was analysed for correlations between iris pigmented lesions and melanoma history.

Results

Cases over the age of 40 had similar numbers of iris pigmented lesions to age matched controls (mean 5.7 vs 5.2, P=0.02), but younger cases and controls had a greater difference (mean 3.96 vs 2.19, P=0.004). A logistic regression adjusted for age, sex, skin, hair and eye colour, skin freckling and naevus count found that the presence of three or more iris pigmented lesions increases the melanoma risk 1.45-fold (CI 95% 1.07-1.95). HERC2/OCA2 rs12913832 and IRF4 rs12203592 influenced both eye colour and the number of iris pigmented lesions. On the HERC2/OCA2 A/A and A/G genotype background there was an increasing proportion of blue eye colour when carrying the IRF4 T allele (P=3x10^-4) and a higher number of iris pigmented lesions with the IRF4 T/T homozygote (P=3x10^-9).

Conclusions

Iris pigmented lesions count provides additional predictive information for melanoma risk above that from conventional risk factors.

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