Isolation and Evaluation of cocktail phages for the control of multi-drug resistant Escherichia coli serotype O104: H4 and E. coli O157: H7 isolates causing diarrhea

Abstract

Escherichia coli serotype O157: H7 and E. coli O104: H4 are well known food borne pathogens causing sever enteric illness. Using bacteriophages as biocontrol agents of some food borne pathogens and multi-drug resistant bacteria has a great attention nowadays. This study aims to test the effect of cocktail phages on the growth of some food borne pathogens and MDR E. coli. Routine conventional PCR was used to confirm the identification of E. coli isolates. Double-layered culture technique was used to isolate phages from sewage water. Morphology of bacteriophage was described using Transmission Electron Microscopy (TEM) and spot test was performed to determine host range of the phage cocktail. Phage Cocktail of Siphoviridae and Podoviridae family infecting E. coli O157: H7, E. coli O104: H4 and untypeable E. coli (neither O157 nor O104) has been isolated from sewage water. Phage cocktail showed both lytic and lysogenic activity. Lytic activity was observed against E. coli O157: H7, E. coli O104: H4 isolates, Staphylococcus. aureus ATCC6538 and Pseudomonas aeruginosa ATCC 10145 while the lysogenic activity was observed against the untypeable strain. The tested phage cocktail showed a promising inhibitory action on E. coli O157: H7 and O104: H4, S. aureus ATCC6538 and P. aeruginosa ATCC 10145, suggesting the possibility of its use as a biocontrol tool or as natural food preservatives for many food products.