A Multiple Closed‐loops Robotic Calibration for Accurate Surgical Puncture

xlomafota13 shared this article with you from Inoreader A Multiple Closed‐loops Robotic Calibration for Accurate Surgical Puncture Via The International Journal of Medical Robotics and Computer Assisted Surgery Abstract Robotic puncture system increasingly demands stringent standard in target location accuracy. The positional and orientational transformation relationships among all components of the system are supposed to be calibrated and identified preoperatively. The target location performance is directly determined by the calibration result. Therefore, a multiple closed‐loops calibration approach is proposed to achieve high‐level calibration accuracy in robotic puncture system. This method takes as input the three‐dimensional position information of the retro‐reflective markers mounted on the surgical tool, which is detected by the optical tracking system in real time during robotic movement. There is less complicated mathematical derivation and calculation in the presented algorithm by applying the closed‐loop principle. Experimental results validate that it can achieve accurate robotic target location with less input data and computation‐cost, satisfying the clinical puncture r equirements. The spatial calibration between robotic arm and optical tracking system efficiently realized by the presented approach present an alternative which can be safely applied to the robotic puncture system for accurate insertion. This article is protected by copyright. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Automatic Detection of Pharyngeal Fricatives in Cleft Palate Speech Using Acoustic Features Based on the Vocal Tract Area Spectrum

xlomafota13 shared this article with you from Inoreader Automatic Detection of Pharyngeal Fricatives in Cleft Palate Speech Using Acoustic Features Based on the Vocal Tract Area Spectrum Via Computer Speech & Language Publication date: Available online 17 February 2021 Source: Computer Speech & Language Author(s): Jia Fu, Fei He, Heng Yin, Ling He View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Refractory lymphorrhoea of the neck treated with etilefrine

xlomafota13 shared this article with you from Inoreader Refractory lymphorrhoea of the neck treated with etilefrine Via European Annals of Otorhinolaryngology, Head and Neck Diseases Publication date: Available online 16 February 2021 Source: European Annals of Otorhinolaryngology, Head and Neck Diseases Author(s): C. Mathis, S. Hamitouche, J.-P. Barberot, P. Lindas View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Developing an enhanced 7-color multiplex IHC protocol to dissect immune infiltration in human cancers

xlomafota13 shared this article with you from Inoreader Developing an enhanced 7-color multiplex IHC protocol to dissect immune infiltration in human cancers Via Head and neck squamous cell carcinoma by Zhaoyu Sun, Richard Nyberg, Yaping Wu, Brady Bernard, William L. Redmond The TSA Opal multiplex immunohistochemistry (mIHC) protocol (PerkinElmer) has been used to characterize immune infiltration in human cancers. This technique allows multiple biomarkers to be simultaneously stained in a single tissue section, which helps to elucidate the spatial relationship among individual cell types. We developed and optimized two improved mIHC protocols for a 7-color panel containing 6 biomarkers (CD3, CD8, CD163, PD-L1, FoxP3, and cytokeratin (CK)) and DAPI. The only difference between these two protocols was the staining sequence of those 6 biomarkers as the first sequence is PD-L1/CD163/CD8/CK/CD3/FoxP3/DAPI and the second sequence is FoxP3/CD163/CD8/CK/CD3/PD-L1/DAPI. By comparing PD-L1/FoxP3 staining in mIHC and singleplex PD-L1/FoxP3 staining on the adjacent slide, we demonstrated that the staining sequence does not affect the staining intensity of individual biomarkers as long as a proper antigen retrieval method was used. Our study suggests that use of an a ntigen retrieval buffer with higher pH value (such as Tris-EDTA pH9.0) than that of the stripping buffers (such as citrate buffer pH6.0) is helpful when using this advanced mIHC method to develop panels with multiple biomarkers. Otherwise, individual biomarkers may exhibit different intensities when the staining sequence is changed. By using this protocol, we characterized immune infiltration and PD-L1 expression in head and neck squamous cell carcinoma (HNSCC), breast cancer (BCa), and non-small cell lung cancer (NSCLC) specimens. We observed a statistically significant increase in CD3+ cell populations within the stroma of NSCLC as compared to BCa and increased PD-L1+ tumor cells in HNSCC as opposed to BCa. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Direct healthcare costs of lip, oral cavity and oropharyngeal cancer in Brazil

xlomafota13 shared this article with you from Inoreader Direct healthcare costs of lip, oral cavity and oropharyngeal cancer in Brazil Via Head and neck cancers by Vanessa Milani, Ana Laura de Sene Amâncio Zara, Everton Nunes da Silva, Larissa Barbosa Cardoso, Maria Paula Curado, Rejane Faria Ribeiro-Rotta The efficiency of public policies includes the measurement of the health resources used and their associated costs. There is a lack of studies evaluating the economic impact of oral cancer (OC). This study aims to estimate the healthcare costs of OC in Brazil from 2008 to 2016. This is a partial economic evaluation using the gross costing top-down method, considering the direct healthcare costs related to outpatients, inpatients, intensive care units, and the number of procedures, from the perspective of the public health sector. The data were extracted from the Outpatient and Inpatient Information System of the National Health System, by diagnosis according to the 10th Revision of the International Classification of Diseases, according to sites of interest: C00 to C06, C09 and C10. The values were adjusted for annual accumulated inflation and expressed in 2018 I$ (1 I$ = R$2,044). Expenditure on OC healthcare in Brazil was I$495.6 million, which was composed of 50.8% (I$2 51.6 million) outpatient and 49.2% (I$244.0 million) inpatient healthcare. About 177,317 admissions and 6,224,236 outpatient procedures were registered. Chemotherapy and radiotherapy comprised the largest number of procedures (88.8%) and costs (94.9%). Most of the costs were spent on people over 50 years old (72.9%) and on males (75.6%). Direct healthcare costs in Brazil for OC are substantial. Outpatient procedures were responsible for the highest total cost; however, inpatient procedures had a higher cost per procedure. Men over 50 years old consumed most of the cost and procedures for OC. The oropharynx and tongue were the sites with the highest expenditure. Further studies are needed to investigate the cost per individual, as well as direct non-medical and indirect costs of OC. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Developing an enhanced 7-color multiplex IHC protocol to dissect immune infiltration in human cancers

xlomafota13 shared this article with you from Inoreader Developing an enhanced 7-color multiplex IHC protocol to dissect immune infiltration in human cancers Via Head and neck cancers by Zhaoyu Sun, Richard Nyberg, Yaping Wu, Brady Bernard, William L. Redmond The TSA Opal multiplex immunohistochemistry (mIHC) protocol (PerkinElmer) has been used to characterize immune infiltration in human cancers. This technique allows multiple biomarkers to be simultaneously stained in a single tissue section, which helps to elucidate the spatial relationship among individual cell types. We developed and optimized two improved mIHC protocols for a 7-color panel containing 6 biomarkers (CD3, CD8, CD163, PD-L1, FoxP3, and cytokeratin (CK)) and DAPI. The only difference between these two protocols was the staining sequence of those 6 biomarkers as the first sequence is PD-L1/CD163/CD8/CK/CD3/FoxP3/DAPI and the second sequence is FoxP3/CD163/CD8/CK/CD3/PD-L1/DAPI. By comparing PD-L1/FoxP3 staining in mIHC and singleplex PD-L1/FoxP3 staining on the adjacent slide, we demonstrated that the staining sequence does not affect the staining intensity of individual biomarkers as long as a proper antigen retrieval method was used. Our study suggests that use of an a ntigen retrieval buffer with higher pH value (such as Tris-EDTA pH9.0) than that of the stripping buffers (such as citrate buffer pH6.0) is helpful when using this advanced mIHC method to develop panels with multiple biomarkers. Otherwise, individual biomarkers may exhibit different intensities when the staining sequence is changed. By using this protocol, we characterized immune infiltration and PD-L1 expression in head and neck squamous cell carcinoma (HNSCC), breast cancer (BCa), and non-small cell lung cancer (NSCLC) specimens. We observed a statistically significant increase in CD3+ cell populations within the stroma of NSCLC as compared to BCa and increased PD-L1+ tumor cells in HNSCC as opposed to BCa. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Role of Transforming Growth Factor‐β1 in Recessive Dystrophic Epidermolysis Bullosa Squamous Cell Carcinoma

xlomafota13 shared this article with you from Inoreader Role of Transforming Growth Factor‐β1 in Recessive Dystrophic Epidermolysis Bullosa Squamous Cell Carcinoma Via Experimental Dermatology Abstract Squamous cell carcinoma (SCC) develops in more than 80% of individuals with the skin blistering disorder recessive dystrophic epidermolysis bullosa (RDEB). In contrast with UV‐induced SCC, RDEB‐SCC results from skin damage and has a high proliferative and metastatic rate with five‐year survival near zero. Our objective is to determine the mechanisms underlying the increased metastatic tendencies of RDEB‐SCC. RDEB‐SCC cultured cell lines were treated with RDEB and non RDEB fibroblast conditioned media and assayed for migration and invasion with and without small molecule inhibitors for TGFβ and other downstream signal transduction pathways. TGFβ1 secreted by RDEB dermal fibroblasts has been found to induce migration and invasion, and to increase expression of epithelial‐to‐mesenchymal transition markers in an RDEB‐SCC line. These effects were reversed upon inhibition of TGFβ signaling and its downstream pathways MEK/ERK, P38 kinase, and SMAD3. A number of small m olecule inhibitors for these pathways are in different phases of various clinical trials and may be applicable to RDEB‐SCC patients. Studying the mechanisms of the extreme form RDEB‐SCC may inform studies of other types of SCC, as well as lead to better therapies for RDEB patients. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Droplet digital PCR of tumor suppressor gene methylation in serial oral rinses of patients with head and neck squamous cell carcinoma

xlomafota13 shared this article with you from Inoreader Droplet digital PCR of tumor suppressor gene methylation in serial oral rinses of patients with head and neck squamous cell carcinoma Via Head & Neck Abstract Background Head and neck squamous cell carcinoma (HNSCC) currently lacks sensitive approaches to detect cancer‐related traits in body fluid. Methods Methylation of tumor suppressor genes (TSGs) (PAX5, EDNRB, and DCC) were measured in the oral rinses from 50 HNSCC and 58 control subjects using droplet digital PCR (ddPCR). Diagnostic accuracies in detecting HNSCC and the detection rate of recurrence in the post‐treatment monitoring were analyzed. Results ddPCR TSG methylation detection in oral rinses for diagnosis of HNSCC had an AUC of 0.892 for PAX5, 0.753 for EDNRB, and 0.729 for DCC. Significant drop of TSG methylation was observed after completion of surgery (p < 0.01). 76.9% of the relapse cases had a pre‐emptive rebound of methylation above presurgery levels in at least one of the tested markers before confirmed recurrence. Conclusions Utilizing ddPCR for TSG methylation detection in oral rinses shows potential for detection and monitoring of HNSCC. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Diagnostic value of exosomal circMYC in radioresistant nasopharyngeal carcinoma: Methodological issues

xlomafota13 shared this article with you from Inoreader Diagnostic value of exosomal circMYC in radioresistant nasopharyngeal carcinoma: Methodological issues Via Head & Neck View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Hepatitis C Virus RNA Transcript Associates with Prognosis in Non‐human Papillomavirus Associated Head and Neck Squamous Cell Carcinoma

xlomafota13 shared this article with you from Inoreader Hepatitis C Virus RNA Transcript Associates with Prognosis in Non‐human Papillomavirus Associated Head and Neck Squamous Cell Carcinoma Via The Laryngoscope Objective/Hypothesis Hepatitis C virus (HCV) was reported to associate with head and neck squamous cell carcinoma (HNSCC) in many studies. However, its correlation with prognosis of non‐human papillomavirus (HPV) associated HNSCC remains unknown. Here, we sought to investigate clinical significance of HCV RNA transcript in non‐HPV associated HNSCC by analyzing corresponding RNA‐seq data. Study Design A retrospective cohort study. Methods Four hundred and forty‐eight non‐HPV associated HNSCC patients with aligned RNA‐seq and clinical follow‐up data were included and divided into two groups: low‐HCV and high‐HCV. Means of continuous variables and proportions of categorical variables were compared using independent sample t‐test and chi‐square test, respectively. Survival data were compared using Cox regression analysis, Kaplan–Meier curves, and log‐rank test. Expression of genome‐wide mRNAs and abundance of immune cells were compared using volcano plot and cell signature estimated score analysis. Results HCV RNA transcript negatively correlates with pathologic (P = .028) and clinical‐stage (P = .023), clinical N stage (P = .025), and nodal extracapsular spread (P = .042) and is an independent prognosis factor in non‐HPV associated HNSCC (HR = 1.488; 95% CI: 1.004–2.206; P = .048). Elevated expression of HCV improved 5‐year overall survival (43.6% vs. 53.2%; P = .035) in all non‐HPV associated HNSCC patients, the same as in male (46.6% vs. 58.7%; P = .049), clinical M0 stage (42.8% vs. 52.9%; P = .036), white (42.9% vs. 55.9%; P = .010), and histologic grade 1 to 2 subgroups (42.1% vs. 57.2%; P = .043). The expression of several immune‐related genes and abundance of some immune cells significantly changed with the increase of HCV RNA transcript, while HCV‐related oncogenes and tumor suppressor gene did not. Conclusions HCV RNA transcript is an independent favorable factor for prognosis of non‐HPV associated HNSCC. Levels of Evidence 4 Laryngoscope, 2021 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Endoscopic Posterior Cricoid Split and Rib Graft Without Tracheostomy: Case and Literature Review

xlomafota13 shared this article with you from Inoreader Endoscopic Posterior Cricoid Split and Rib Graft Without Tracheostomy: Case and Literature Review Via The Laryngoscope Endoscopic posterior cricoid split and rib grafting (EPCS/RG) for the treatment of posterior laryngeal stenosis has some advantages over traditional open approaches, including improved surgical visualization and decreased morbidity. Many pediatric patients who undergo EPCS/RG have indwelling tracheostomy, which may be utilized to help manage the airway perioperatively. The role for de novo tracheostomy placement at the time of EPCS/RG is less clear. We present three cases from a tertiary children's hospital in which EPCS/RG was safely performed without tracheostomy. For patients with posterior laryngeal stenosis but without tracheostomy, EPCS/RG with endotracheal tube stenting might be a safe option. Laryngoscope, 2021 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Evidence-Based Use of Levothyroxine/Liothyronine Combinations in Treating Hypothyroidism: A Consensus Document

xlomafota13 shared this article with you from Inoreader Evidence-Based Use of Levothyroxine/Liothyronine Combinations in Treating Hypothyroidism: A Consensus Document Via liebertpub1 Thyroid, Volume 31, Issue 2, Page 156-182, February 2021. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.