The association between lncRNA H19 and EZH2 expression in patients with EBV-positive laryngeal carcinoma

xlomafota13 shared this article with you from Inoreader The association between lncRNA H19 and EZH2 expression in patients with EBV-positive laryngeal carcinoma Via Acta Otorhinolaryngol Ital Acta Otorhinolaryngol Ital. 2021 Nov 26. doi: 10.14639/0392-100X-N1527. Online ahead of print. ABSTRACT OBJECTIVE: Laryngeal cancer is the second most common malignancy in the head and neck, with Epstein-Barr virus infection as a risk factor. Our aim is to evaluate correlations between the expression of lncRNA H19 and EBV infection in laryngeal cancer and H19 involvement in neoplastic progression through EZH2 association. MATERIALS AND METHODS: 30 paired laryngeal tiss ue specimens (neoplastic and non-neoplastic) were included in the study. Nucleic acid isolation and cDNA synthesis was performed according to the manufacturer's protocol. EBV DNA and expression of lytic (BZLF1) and latent (LMP1) forms of infection were assessed in PCR assays; expression levels of H19 and EZH2 were quantified in qRT-PCR. Data was analysed using GraphPad Prism 5.0. RESULTS: Higher H19 relative expression in neoplastic vs paired non-neoplastic samples was found (p < 0.0001) with a significant increase in EBV DNA positive neoplasms (p = 0.0434). An inverse correlation between H19 and EZH2 expression levels was noticed in EBV positive cases. Additionally, increased levels of H19 in LMP1 positive samples compared with those positive for BZLF1 was found (p = 0.0593). CONCLUSIONS: lncRNA H19 and EZH2 significantly contribute to the development of laryngeal carcinoma, being correlated with EBV infection markers. PMID:34825669 | DOI:10.14639/0392-100X-N1527 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.