Exploring a possibility of using Raman spectroscopy for detection of Lyme disease

xloma.fota13 shared this article with you from Inoreader Exploring a possibility of using Raman spectroscopy for detection of Lyme disease Via Journal of Biophotonics Abstract Lyme disease (LD), one of the most prevalent tick‐borne diseases in the United States (US), is caused by Borreliella burgdorferi sensu stricto. To date, in the US, LD diagnostics is primarily based on a validated two‐tiered serological testing, which overall exhibits low sensitivity among other drawbacks. In the present study, a potential of Raman spectroscopy (RS) to detect Bb infection in mice has been explored. For that, C3H mice were infected with wild‐type Bb strains, 297, B31, or B31‐derived mutant, ∆vlsE. Blood samples taken prior to and post Bb infection were subjected to RS. The data demonstrated that RS did not directly detect Bb spirochetes in blood, but rather sensed biochemical changes associated with Bb infection. Despite Bb infection‐associated blood changes detectable by RS were very limited, the partial least square discriminant analysis showed that the average true positive rates were 86% for 297 and 89% for B31 and ∆vlsE. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.