The major challenge of allergy diagnosis lies in the development of accessible and reliable diagnostics allowing correct prediction of the clinical outcome upon exposure to the offending allergen(s) and cross-reactive structures. Since the late nineties, evidence has accumulated that flow-assisted analysis and quantification of ex vivo activated basophils (BAT) might meet this requirement for different IgE-dependent allergies and particular forms of auto-immune urticaria. Other, so called non-diagnostic applications of the BAT involve therapeutic monitoring, follow-up of natural histories and identification of allergenic recognition sites.
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