LB1537 Matrix metalloproteinase 1 deficiency in epidermal keratinocytes makes them susceptible to IFN-γ and impairs their migration

The hyperproliferative human epidermal keratinocytes HaCaT are often used in experimental studies of psoriasis. Recently, we reported that Th1 cytokines TNF, IFN-γ and IL17 exhibited an antiproliferative effect on HaCaT cells. The aim of this paper was to explore how knocking matrix metalloproteinase 1 (MMP1) down in epidermal keratinocytes affected their proliferation, migration and differentiation and be beneficial for psoriasis. MMP1-deficient and control cells were generated by lentiviral transduction.