Seed priming with Se mitigates As-induced phytotoxicity in rice seedlings by enhancing essential micronutrient uptake and translocation and reducing As translocation

Abstract

We laid down this investigation to explore the promotive and antagonistic aspect of selenium (Se) when supplemented through seed priming technology in rice before sowing into arsenic (As) free and As spiked soil. Findings suggest that As stress inhibits germination (35.38%), seedling growth (38.19%), chlorophyll content by 42.31%, and reduced translocation of iron, zinc, manganese by 19.40, 17.33, and 18.40% respectively, in the seedlings of unprimed seeds. Seedlings of unprimed seeds also had greater As translocation into the aerial part beside repressing micronutrient translocation, significantly. On the contrary, Se-primed seeds had higher germination (27.82%), longer root length (20.14%), greater chlorophyll content beside having greater translocation of iron, zinc, manganese in shoots along with restricting As translocation in rice seedlings by confining more As in the root, in a significant manner (p < 0.05 level) than the unprimed seedlings grown in identical stress. On the other hand, seedlings of Se-primed seeds grown alike the control also had higher germination % (7.08%), root and shoot length with significantly less proline, and hydrogen peroxide content in root and shoot. Findings indicate that seed priming with Se executes dual role, a growth promoting and antagonism in a more practical and farmer-friendly way to mitigate As-induced toxicity and enhance growth in rice seedlings.