Growth, physiological adaptation, and NHX gene expression analysis of Iris halophila under salt stress

Abstract

This study investigated the growth, physiological changes, and the transcript levels of NHX1 gene of Iris halophila in response to low NaCl concentration (50 mM) and high NaCl concentration (150 mM). Our results showed that both 50 and 150 mM NaCl had no obvious negative effects on plant growth; what is more, low NaCl concentration (50 mM) increased root length, root fresh weight, and the ratio of root length to leaf length compared with the control group. The malondialdehyde (MDA) contents in leaves and roots of I. halophila had no obvious difference as compared with control. Proline levels of I. halophila exhibited basically an enhancement under salt stress conditions. Particularly at 4 days, the proline contents in leaves reached 1.85 to 2.31-fold higher and the contents in roots reached 1.27 to 1.62-fold higher than that of control at 50 and 150 mM NaCl, respectively. The contents of the soluble sugar in leaves and roots of I. halophila under 150 mM NaCl at 7 days were 32.4 and 98.7% higher than that of control, respectively. The increase rate of K⁺ contents with the increasing concentration of salt was less than that of Na⁺ contents, but K⁺ contents in the seedlings under NaCl stress was still higher than Na⁺ contents and the ratio of K⁺ to Na⁺ was also greater than 1. The transcript levels of IhNHX1 in leaves of I. halophila at 4 and 7 days under 150 mM NaCl were higher than that of control; however, the transcript levels of IhNHX1 in roots had no significant difference compared with the control under low and high salt stress at 1, 4, and 7 days. Therefore, salt tolerance in I. halophila could be partially due to higher proline, soluble sugar, and K⁺ accumulation.