Cadmium bioaccumulation and antioxidant enzyme activity in hepatopancreas, kidney, and stomach of invasive apple snail Pomacea canaliculata

Abstract

The acute toxicity of Cd was tested, and metal bioaccumulation in tissue was determined for the alien invasive species Pomacea canaliculata and its native competitor Sinotaia quadrata under experimental conditions. The invasive species was more tolerant to Cd toxicity than native species, for which the LC₅₀ values were 4.26, 2.24, and 1.98 mg/L at exposure times of 48, 72, and 96 h, respectively, approximately three times higher than those of the native snails. The viscera accumulated the highest Cd concentration, followed by the foot and shell in both species. Metal concentrations in the above three tissues of P. canaliculata were much higher than those of S. quadrata, irrespective of Cd dose and exposure time. For P. canaliculata, the highest concentration of metal was further observed in the hepatopancreas (0.64–3.98 mg/g) followed by the kidney (0.067–3.78 mg/g), with lowest levels in the stomach (0.062–1.53 mg/g). Among the five antioxidant enzymes, the most responsive enzymes were CAT, ALP, and GST in the hepatopancreas; CAT, POD, and GST in the kidney; and POD in the stomach of exposed animals. These results, demonstrating a high Cd tolerance, may partly explain the ability of P. canaliculata to displace S. quadrata in Cd-contaminated habitat. The Cd was accumulated mainly in the hepatopancreas and kidney of invasive species, which changed the activity of antioxidant enzymes allowing the animals to cope with the toxicity.

Graphical abstract

Cadmium bioaccumulation and antioxidant enzyme activity in the invasive Pomacea canaliculata.