Impact of Ultraviolet Radiation on Expression of Transforming Growth Factor-beta, Smad2, Metalloproteinases, Cathepsin K and Progerin

Abstract

Ultraviolet radiation (UVR) is one of the most important environmental factors involved in photoaging. Exposure to UVR leads to dysregulation of expression of cell-cycle-related proteins which play key role in skin photodegradation that pretends to develop carcinogenesis. This study examines the role of various UVB doses on the expression of transforming growth factor beta (TGF-β), Smad2, cathepsin K, progerin and matrix metalloproteinases (MMPs)-1,-3,-8,-9. A group consisting of 63 healthy individuals underwent one of the following treatments: 1) whole-body exposed to UVB irradiation on each of 10 consecutive days with 0.7 MED, or 2) whole-body irradiation as described followed by a single erythemal UVB dose on a small body area, or 3) irradiated only with a single erythemal UVB dose on small body area, or 4) were not irradiated at all (control group). When we compared all irradiated groups to the control group there was significantly higher expression of TGF-β, MMP-1,-3,-9, and cathepsin K proteins evaluated by western blot method. The results suggest the role of UVB in impairment of proteins expression that is involved in cell cycle's regulation. Changes of the proteins expression involved by acute and chronic UVR confirms its essential role in skin photodestruction. Moreover, obtained result indicates the tendency to occurrence of photoadaptation phenomenon.

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