Diagnostic and prognostic value of Beclin 1 expression in melanoma: a meta-analysis

xlomafota13 shared this article with you from Inoreader Diagnostic and prognostic value of Beclin 1 expression in melanoma: a meta-analysis Via Melanoma Research - Published Ahead-of-Print Autophagy plays a complicated role in the occurrence and development of cancer. Beclin 1 is a significant autophagy-related protein that plays an essential role in tumorigenesis, but its expression is controversial in melanoma. In this meta-analysis, we searched seven studies involving 638 melanoma patients. PubMed, Web o f Science, Google Scholar, Elsevier, and Chinese National Knowledge Infrastructure were used for literature retrieval. The I2 index was used to assess heterogeneity. The expression of Beclin 1 in the primary melanoma group was significantly lower than the non-tumor group tissues (P 0.05). Different expression intensities of Beclin 1 did not affect the overall survival and disease-free survival of melanoma patients. This study showed a trend of low expression of Beclin 1 in melanoma; patients with low expression of Beclin 1 were prone to the possibility of distant metastasis. The inconsistent profile of Beclin 1 expression in the prognosis of melanoma patients warrants further clinical investigation. Received 8 May 2021 Accepted 12 August 2021 Correspondence to Ping Huang, MD, Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jiefang Road, Wuhan, Hubei 430030, China, Tel: +86 15807192632; e-mail: huangping_hust@hotmail.com Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Novel strategy for applying hierarchical density-based spatial clustering of applications with noise towards spectroscopic analysis and detection of melanocytic lesions

xlomafota13 shared this article with you from Inoreader Novel strategy for applying hierarchical density-based spatial clustering of applications with noise towards spectroscopic analysis and detection of melanocytic lesions Via Melanoma Research - Published Ahead-of-Print Advancements in dermoscopy techniques have elucidated identifiable characteristics of melanoma which revolve around the asymmetrical constitution of melanocytic lesions consequent of unfettered proliferative growth as a malignant lesion. This study explores the applications of hierarchical density-based spatial clustering of applications with noise (HDBSCAN) in terms of the direct diagnostic implications of applying agglomerative clustering in the spectroscopic analysis of malignant melanocytic lesions and benign dermatologic spots. 100 images of benign (n = 50) and malignant moles (n = 50) were sampled from the International Skin Imaging Collaboration Archive and processed through two separate Python algorithms. The first of which deconvolutes the three-digit tupled integer identifiers of pixel color in image composition into three separate matrices corresponding to the red, green and blue color channel. Statistical characterization of integer variance was utilized to determine the optimal channel for comparative analysis between malignant and benign image groups. The second applies HDBSCAN to the matrices, identifying agglomerative clustering in the dataset. The results indicate the potential diagnostic applications of HDBSCAN analysis in fast-processing dermoscopy, as optimization of cluster ing parameters according to a binary search strategy produced an accuracy of 85% in the classification of malignant and benign melanocytic lesions. Received 21 May 2021 Accepted 16 July 2021 Correspondence to Jason Yuan Ye, E-mail: jason.ye9@gmail.com This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Evaluation of the whole auditory pathway using high-resolution and functional MRI at 7T parallel-transmit

xlomafota13 shared this article with you from Inoreader Evaluation of the whole auditory pathway using high-resolution and functional MRI at 7T parallel-transmit Via Meniere disease by Sandra Da Costa, Jérémie Clément, Rolf Gruetter, Özlem Ipek Purpose The aim of the present study is to show a MR procedure for the evaluation of simultaneous left and right auditory functions with functional MRI, and high-resolution acquisition of anatomical auditory pathway using parallel-transmit (pTx) methods at 7T. Methods The time-efficient MR acquisition included two steps: RF weights were optimized for the regions-of-interest and high-resolution MR images of the inner-ear were acquired for the first 30 min (400 μm-iso resolution) followed by functional MRI acquisitions along the whole auditory pathway during the next 20 minutes. Data was processed with a linear cross-correlation analysis to define frequency preferences for each voxel in the auditory relays. Results Tonotopic maps revealed ordered bilateral frequency gradients in the auditory relays whereas at the level of the cochlear nuclei and superior olivary complexes the frequency gradients were less evident. A 21% increase in transmit-field efficiency was achiev ed over the left/right inner-ear regions and thus its main structures were clearly discernible using the pTx methods, compared to a single transmit RF coil. Conclusion Using 7T pTx allows a fast (less than 60 min in total) and qualitative evaluation of the simultaneous left and right auditory response along the entire auditory pathway, together with high-resolution anatomical images of the inner-ear. This could be further used for patient examination at 7T. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Free vascularized fascia lata flap for total columella reconstruction

xlomafota13 shared this article with you from Inoreader Free vascularized fascia lata flap for total columella reconstruction Via American Journal of Otolaryngology Publication date: Available online 11 September 2021 Source: American Journal of Otolaryngology Author(s): Rachel E. Aliotta, Joseph Meleca, Allison Vidimos, Michael A. Fritz View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Association of Bethesda category and molecular mutation in patients undergoing thyroidectomy

xlomafota13 shared this article with you from Inoreader Association of Bethesda category and molecular mutation in patients undergoing thyroidectomy Via Clinical Otolaryngology Abstract Objectives The aim of this study was to ascertain the relationship between Bethesda category and molecular mutation of thyroid nodules in patients undergoing thyroidectomy. Design A retrospective cohort of patients who underwent thyroidectomy following needle biopsy and molecular profile testing was performed. Setting Two tertiary care academic hospitals. Participants Consecutive patients with a dominant thyroid nodule who underwent both USFNA and molecular profile testing followed by thyroidectomy were included in the study. Main Outcome and Measures The main outcome was postoperative diagnosis of thyroid cancer and aggressivity of disease based on histopathological variants, nodal metastasis or extra-thyroidal extension. Associations between Bethesda category, molecular mutation and postoperative pathology was assessed using descriptive analysis and Chi-square testing. Results 451 patients were included. 95.9% (93/97) of patients with a BRAFV600E mutation had a Bethesda category V or VI (P<0.001), and all had confirmed thyroid cancer on postoperative pathology. Those with H, K or N RAS or EIF1AX mutations, gene expression profiling (GEP) or copy number alterations showed an association with Bethesda categories III and IV (P≤0.01). Those with no identified molecular mutation had a lower incidence of aggressive thyroid cancer compared to those with an identified mutation (12.6% vs 44.3%, P<0.01). Conclusion BRAFV600E mutations were associated with thyroid cancer subtypes known to be more aggressive whereas RAS and EIF1AX mutations, copy number alterations, and GEP were related to Bethesda categories III and IV. These findings may help thyroid specialists better identify aggressive thyroid nodules associated with indeterminate Bethesda categories. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

SARS‐CoV‐2 tracheitis in laryngectomized patients: a consecutive case‐series study

xlomafota13 shared this article with you from Inoreader SARS‐CoV‐2 tracheitis in laryngectomized patients: a consecutive case‐series study Via Clinical Otolaryngology Abstract SARS-CoV-2 is a possible cause of acute severe tracheitis in laryngectomees. In our series, the clinical picture was characterized by a haemorrhagic tracheitis with a slow resolution pattern. We observed a histological pattern of erosive inflammation of the respiratory epithelium. Planned tracheo-bronchoscopy and tracheal toilettes are recommended to prevent critical obstruction of the airway, which can be fatal in patients with associated impairment of lung function caused by SARS-CoV-2 infection. The present cases highlight the need for close interdisciplinary working and communication in the management of airway complications of COVID-19 infection. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

A Novel Two Surgeons Approach to Bronchial Foreign Body Removal by Optical Instruments- An 11 Years Institutional Experience

xlomafota13 shared this article with you from Inoreader A Novel Two Surgeons Approach to Bronchial Foreign Body Removal by Optical Instruments- An 11 Years Institutional Experience Via Otolaryngology Indian Abstract A potentially life-threatening emergency in ENT practice is the aspiration of tracheobronchial foreign body. This commonly affects young children and requires early extraction. Successful removal provides immediate relief. However procedural complications can cause devastating morbidity like anoxic encephalopathy or instant death. The aim of this study is to describe the experience in our institution with two surgeons approach for removal of tracheobronchial foreign bodies by telescope and optical forceps. This is an observational study from the period of March 2010 to April 2021 in which 96 patients (59 males, 37 females; age range 3 months to 15 years) were diagnosed with foreign body aspiration. In all cases rigid bronchoscopy and FB removal under general anaesthesia was performed using the two surgeons technique. This technique was instituted once Storz make paediatric telescope (2.9 mm diameter 36 cm length), optical grasping forceps and optical suction were procured in March 2010. Aspirated material was inorganic or organic with varying location in right main bronchus, left main bronchus or the trachea. We highlight the OT setup, surgical algorithm and the advantages of this technique. Visual and tactile coordination between the two surgeons being the crux of this procedure; allows hands on mentoring of the next generation of budding surgeons in a real-life scenario without compromising patient safety. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Bone Graft Erosion into Hypopharynx Presenting with Stridor: Rare Late Complication Following Cervical Spine Surgery

xlomafota13 shared this article with you from Inoreader Bone Graft Erosion into Hypopharynx Presenting with Stridor: Rare Late Complication Following Cervical Spine Surgery Via Otolaryngology Indian Cannot fetch mobilized content. Please try again later. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Effect of Efferent Stimulation on the Differential Sensitivity in Individuals with Normal Hearing

xlomafota13 shared this article with you from Inoreader Effect of Efferent Stimulation on the Differential Sensitivity in Individuals with Normal Hearing Via Otolaryngology Indian Abstract The aim of the article was to compare the conditions of silent and contralateral noise on Differential sensitivity in normal hearing individuals. A total of 40 participants (20 males and 20 females) were enrolled in the study with a mean age of 21.7 years, participants with normal hearing thresholds and no history of middle ear pathology were enrolled for the study. Difference limen tests such as difference limen for intensity (DLI), difference limen for frequency (DLF), and difference limen for time (DLT) were carried out in these 40 individuals in the two conditions of silent and contralateral noise using MATLAB. Statistical analysis was carried out using the SPSS version 25.0 were descriptive and inferential statistics were carried out. Data was normally distributed on the Shapiro–Wilk's test of normality due to which a paired t test was carried out to establish the nature of significance between the silent and contralateral noise condition. Results r eveal the presence of significant difference (P < 0.01) between the groups for DLF and DLT with contralateral noise condition performing better than silent condition for the parameters. However, no significant difference was obtained for DLI. There is a positive effect of the efferent auditory pathway on the Differential sensitivity thus implying that speech perception in noise is improved in the presence of background noise for normal hearing individuals due to this effect. But in case of DLI, the reduced spread of excitation could be the reason why there were no significant differences between silent and contralateral noise condition. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Outcomes of salvage treatment in patients with recurrent oral squamous cell carcinoma

xlomafota13 shared this article with you from Inoreader Outcomes of salvage treatment in patients with recurrent oral squamous cell carcinoma Via Head & Neck Abstract Background The survival outcomes of different salvage treatments for patients with recurrent oral cancer remain unclear. Methods A total of 556 patients with recurrent oral cancer between 2010 and 2015 were reviewed. Clinical/pathological risk factors and different salvage treatments were analyzed. Results The 2-year disease-free survival rates after recurrence in patients not receiving salvage operation (305 patients), receiving salvage operation with (121 patients), and without (130 patients) major pathological risk factors (margin or extranodal extension) were 5.3%, 32.4%, and 77.2%, respectively (p < 0.001). The 2-year overall survival rates were 20.3%, 58.4%, and 89.2%, respectively (p < 0.001). A late-onset recurrence, salvage radiation, and salvage operation were independent factors for good disease-free and overall survival. Salvage radiation showed survival benefits among patients not indicated for salvage operations. Conclusions Salvage operation was the first choice for recurrent oral cancer. Patients who received the salvage operation without major risk factors had the best survival. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Transverse cervical artery anterior perforator flap for head and neck oncological reconstruction: Preliminary study

xlomafota13 shared this article with you from Inoreader Transverse cervical artery anterior perforator flap for head and neck oncological reconstruction: Preliminary study Via Head & Neck Abstract Background To describe the indications, technique, and our experience in the application of the transverse cervical artery anterior perforator flap (ap-TCAF) for reconstruction of head and neck oncological defects. Methods From September 2016 to September 2019, 11 patients underwent surgical treatment for head neck squamous cell carcinoma and were subsequently reconstructed with ap-TCAFs. The clinical details were recorded, and the postoperative appearance and function were analyzed. Results The ap-TCAF was used to reconstruct intraoral defects in eight patients and to repair an oropharyngeal defect in one patient. In two remaining patients, the ap-TCAF was divided into two to restore defects with both an intraoral and extraoral component. The flap size ranged from 6 × 4 cm to 15 × 9 cm. All flaps healed uneventfully. There was no delayed wound healing or dysfunction at the donor site. Conclusions The ap-TCAF is reliable with characteristics making it useful for head and neck oncological reconstruction, especially in male patients. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.

Non‐invasive evaluation of subjective sensitive skin by transcriptomics using mRNA in skin surface lipids

xlomafota13 shared this article with you from Inoreader Non‐invasive evaluation of subjective sensitive skin by transcriptomics using mRNA in skin surface lipids Via Experimental Dermatology Abstract Sensitive skin is a condition characterized by hypersensitivity to environmental stimuli, and its pathophysiology has not been fully elucidated. Questionnaires based on subjective symptoms, intervention tests, and measuring devices are used to diagnose sensitive skin; however, objective evaluation methods, including biomarkers, remain to be established. This study aimed to investigate the molecular profiles of self-reported sensitive skin, understand its pathophysiology, and explore its biomarkers. Here, we analyzed RNAs in skin surface lipids (SSL-RNAs), which can be obtained non-invasively by wiping the skin surface with an oil-blotting film, to compare the transcriptome profiles between questionnaire-based 'sensitive' (n = 11) and 'non-sensitive' (n = 10) skin participants. Exactly 417 differentially expressed genes in SSL-RNAs from individuals with sensitive skin were identified, of which C-C motif chemokine ligand 17 and interferon-γ pathways were elevated, while 50 olfactory receptor (OR) genes were downregulated. The expression of the detectable 101 OR genes was lower in individuals with sensitive skin compared to that in those with non-sensitive skin and was particularly associated with the subjective sensitivity among skin conditions. The receiver operating characteristic (ROC) curve demonstrated that the mean expression levels of OR genes in SSL-RNAs could discriminate subjective skin sensitivity with an area under the ROC curve of 0.836. SSL-RNA profiles suggest a mild inflammatory state in sensitive skin, and overall OR gene expression could be a potential indicator for sensitive skin. View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.