Osteoblasts-derived exosomes regulate osteoclast differentiation through miR-503-3p/Hpse axis

xlomafota13 shared this article with you from Inoreader Osteoblasts-derived exosomes regulate osteoclast differentiation through miR-503-3p/Hpse axis Via histochem Acta Histochem. 2021 Sep 27;123(7):151790. doi: 10.1016/j.acthis.2021.151790. Online ahead of print. ABSTRACT MicroRNAs (miRNAs) are involved in bone remodeling by regulating the balance of bone formation and resorption. Increasing evidence has confirmed that the communication between osteoclast and osteoblast through secreting exosomes and transferring miRNAs. It has been reported that mineralized osteoblasts release exosomes containing more miR-503-3p. However, the roles a nd molecular mechanisms of osteoblast exosomes-derived miR-503-3p in osteoclast differentiation remain elusive. Here, we isolated exosomes from the supernatant of osteoblasts and identified the exosome characterization through transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blot assay. In addition, we found that exosomes and miR-503-3p secreted by osteoblasts inhibited the differentiation of osteoclast progenitor cells. Meanwhile, we found that Hpse (heparanase gene) was a target gene of miR-503-3p and miR-503-3p inhibited the osteoclast differentiation through downregulating the expression of Hpse. In summary, our results demonstrated the roles and the mechanism of osteoblast-derived exosomes inhibited the osteoclast differentiation via miR-503-3p/Hpse axis. PMID:34592492 | DOI:10.1016/j.acthis.2021.151790 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.