Abstract
Objective
To investigate the role of human papillomavirus (HPV) in laryngeal squamous cell carcinoma (LSCC) and analyze the relationship between HPV and the expression of cell cycle‐related proteins.
Design
The study consisted of LSCC between 2005 and 2011 in Tongren Hospital. Clinical data such as age, sex, smoking/ alcohol consumption and TNM stage were collected. HPV DNA and cell cycle‐related proteins were assessed in terms of clinical features.
Setting
Single center study.
Participants
A total of 332 LSCC patients were included in the study.
Main outcome measures
The presence of genotype‐specific HPV DNA was evaluated using PCR‐RDB in formalin‐fixed paraffin‐embedded tissues. All samples were also evaluated for p16INK4A, p21WAF1/CIP1, P53, Cyclin D1, and Ki67 immunohistochemical staining by tissue microarray.
Results
HPV DNA was detected in 45 of 332 (13.55%) patients with LSCC, with HPV‐16 being the predominant genotype. The presence of HPV‐16 DNA was significantly associated with basaloid squamous cell carcinoma and cystic lymph node metastasis (P < 0.05). Of the 332 patients 36 (10.84%) were scored as p16INK4A positivity, and they were more likely to be female (P < 0.05). Cyclin D1‐positivity and p21WAF1/CIP1‐positivity were observed in 60.24% (200/332)and 40.66%(135/332), respectively. In 114 cases (34.33%), LSCCs had moderate‐ to ‐strong p53 accumulation, which was correlated with TNM stage (P < 0.05). HPV‐16 DNA was correlated with p16INK4A, and manifested a higher Ki‐67 labeling index and p21WAF1/CIP1 expression than HPV‐16‐negative tumors (P < 0.05). No relationship was observed between Cyclin D1or P53 expression and HPV‐16 infection (P > 0.05).
Conclusion
HPV DNA was detected in 13.55% patients with LSCC, with HPV‐16 being the predominant genotype and it was correlated with p16INK4A, and manifested a higher Ki‐67 labeling index and p21WAF1/CIP1 expression than HPV‐16‐negative tumors.
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