Phytoremediation of Mn-contaminated paddy soil by two hyperaccumulators ( Phytolacca americana and Polygonum hydropiper ) aided with citric acid

Abstract

The purpose of this study was to investigate the phytoremediation potential of two hyperaccumulator plants, Phytolacca americana L. and Polygonum hydropiper L., on manganese-contaminated paddy soils. The biomass growth, Mn concentrations in plant tissues, and potential Mn removal efficiency from soils of these two plants were studied with citric acid, and the mechanisms of citric acid on these two plants were analyzed by examining the root activity, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in leaves, as well as the concentrations of O₂·⁻ and H₂O₂ in leaves. The results showed that the biomass of these two plants were both promoted under low level of citric acid (3 mmol kg⁻¹). The concentration of Mn in the plants and the amount of Mn removed from the soil by the plants through harvesting were enhanced at low and intermediate (10 mmol kg⁻¹) citric acid application levels. The results also showed that root activity was enhanced at the low citric acid level and significantly inhibited under the intermediate and high levels (15 mmol kg⁻¹), which indicates the facilitative function of the low level of citric acid and the inhibitive function of the high level of citric acid application on plant biomass growth. Under the low and intermediate levels of citric acid application, O₂·⁻ in the plant leaves increased sharply, and the SOD, POD, and CAT activities also increased sharply, which made the level of H₂O₂ very similar to that of the control, ensuring the health of the plants. At the high level of citric acid application, however, the O₂·⁻ continued to rise sharply, while the activity of the three antioxidant enzymes declined sharply, causing the concentration of hydrogen peroxide to be much higher than that in the control, thus endangering the plants. The present study shows the potential of P. hydropiper for use in the phytoremediation of soil contaminated with a relatively low level of manganese.